Two way ANOVA - overview
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Two way ANOVA | Two sample $t$ test - equal variances assumed | Spearman's rho | Paired sample $t$ test | Two sample $z$ test |
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Independent/grouping variables | Independent/grouping variable | Variable 1 | Independent variable | Independent/grouping variable | |
Two categorical, the first with $I$ independent groups and the second with $J$ independent groups ($I \geqslant 2$, $J \geqslant 2$) | One categorical with 2 independent groups | One of ordinal level | 2 paired groups | One categorical with 2 independent groups | |
Dependent variable | Dependent variable | Variable 2 | Dependent variable | Dependent variable | |
One quantitative of interval or ratio level | One quantitative of interval or ratio level | One of ordinal level | One quantitative of interval or ratio level | One quantitative of interval or ratio level | |
Null hypothesis | Null hypothesis | Null hypothesis | Null hypothesis | Null hypothesis | |
ANOVA $F$ tests:
| H0: $\mu_1 = \mu_2$
Here $\mu_1$ is the population mean for group 1, and $\mu_2$ is the population mean for group 2. | H0: $\rho_s = 0$
Here $\rho_s$ is the Spearman correlation in the population. The Spearman correlation is a measure for the strength and direction of the monotonic relationship between two variables of at least ordinal measurement level. In words, the null hypothesis would be: H0: there is no monotonic relationship between the two variables in the population. | H0: $\mu = \mu_0$
Here $\mu$ is the population mean of the difference scores, and $\mu_0$ is the population mean of the difference scores according to the null hypothesis, which is usually 0. A difference score is the difference between the first score of a pair and the second score of a pair. | H0: $\mu_1 = \mu_2$
Here $\mu_1$ is the population mean for group 1, and $\mu_2$ is the population mean for group 2. | |
Alternative hypothesis | Alternative hypothesis | Alternative hypothesis | Alternative hypothesis | Alternative hypothesis | |
ANOVA $F$ tests:
| H1 two sided: $\mu_1 \neq \mu_2$ H1 right sided: $\mu_1 > \mu_2$ H1 left sided: $\mu_1 < \mu_2$ | H1 two sided: $\rho_s \neq 0$ H1 right sided: $\rho_s > 0$ H1 left sided: $\rho_s < 0$ | H1 two sided: $\mu \neq \mu_0$ H1 right sided: $\mu > \mu_0$ H1 left sided: $\mu < \mu_0$ | H1 two sided: $\mu_1 \neq \mu_2$ H1 right sided: $\mu_1 > \mu_2$ H1 left sided: $\mu_1 < \mu_2$ | |
Assumptions | Assumptions | Assumptions | Assumptions | Assumptions | |
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Test statistic | Test statistic | Test statistic | Test statistic | Test statistic | |
For main and interaction effects together (model):
| $t = \dfrac{(\bar{y}_1 - \bar{y}_2) - 0}{s_p\sqrt{\dfrac{1}{n_1} + \dfrac{1}{n_2}}} = \dfrac{\bar{y}_1 - \bar{y}_2}{s_p\sqrt{\dfrac{1}{n_1} + \dfrac{1}{n_2}}}$
Here $\bar{y}_1$ is the sample mean in group 1, $\bar{y}_2$ is the sample mean in group 2, $s_p$ is the pooled standard deviation, $n_1$ is the sample size of group 1, and $n_2$ is the sample size of group 2. The 0 represents the difference in population means according to the null hypothesis. The denominator $s_p\sqrt{\dfrac{1}{n_1} + \dfrac{1}{n_2}}$ is the standard error of the sampling distribution of $\bar{y}_1 - \bar{y}_2$. The $t$ value indicates how many standard errors $\bar{y}_1 - \bar{y}_2$ is removed from 0. Note: we could just as well compute $\bar{y}_2 - \bar{y}_1$ in the numerator, but then the left sided alternative becomes $\mu_2 < \mu_1$, and the right sided alternative becomes $\mu_2 > \mu_1$. | $t = \dfrac{r_s \times \sqrt{N - 2}}{\sqrt{1 - r_s^2}} $ Here $r_s$ is the sample Spearman correlation and $N$ is the sample size. The sample Spearman correlation $r_s$ is equal to the Pearson correlation applied to the rank scores. | $t = \dfrac{\bar{y} - \mu_0}{s / \sqrt{N}}$
Here $\bar{y}$ is the sample mean of the difference scores, $\mu_0$ is the population mean of the difference scores according to the null hypothesis, $s$ is the sample standard deviation of the difference scores, and $N$ is the sample size (number of difference scores). The denominator $s / \sqrt{N}$ is the standard error of the sampling distribution of $\bar{y}$. The $t$ value indicates how many standard errors $\bar{y}$ is removed from $\mu_0$. | $z = \dfrac{(\bar{y}_1 - \bar{y}_2) - 0}{\sqrt{\dfrac{\sigma^2_1}{n_1} + \dfrac{\sigma^2_2}{n_2}}} = \dfrac{\bar{y}_1 - \bar{y}_2}{\sqrt{\dfrac{\sigma^2_1}{n_1} + \dfrac{\sigma^2_2}{n_2}}}$
Here $\bar{y}_1$ is the sample mean in group 1, $\bar{y}_2$ is the sample mean in group 2, $\sigma^2_1$ is the population variance in population 1, $\sigma^2_2$ is the population variance in population 2, $n_1$ is the sample size of group 1, and $n_2$ is the sample size of group 2. The 0 represents the difference in population means according to the null hypothesis. The denominator $\sqrt{\frac{\sigma^2_1}{n_1} + \frac{\sigma^2_2}{n_2}}$ is the standard deviation of the sampling distribution of $\bar{y}_1 - \bar{y}_2$. The $z$ value indicates how many of these standard deviations $\bar{y}_1 - \bar{y}_2$ is removed from 0. Note: we could just as well compute $\bar{y}_2 - \bar{y}_1$ in the numerator, but then the left sided alternative becomes $\mu_2 < \mu_1$, and the right sided alternative becomes $\mu_2 > \mu_1$. | |
Pooled standard deviation | Pooled standard deviation | n.a. | n.a. | n.a. | |
$ \begin{aligned} s_p &= \sqrt{\dfrac{\sum\nolimits_{subjects} (\mbox{subject's score} - \mbox{its group mean})^2}{N - (I \times J)}}\\ &= \sqrt{\dfrac{\mbox{sum of squares error}}{\mbox{degrees of freedom error}}}\\ &= \sqrt{\mbox{mean square error}} \end{aligned} $ | $s_p = \sqrt{\dfrac{(n_1 - 1) \times s^2_1 + (n_2 - 1) \times s^2_2}{n_1 + n_2 - 2}}$ | - | - | - | |
Sampling distribution of $F$ if H0 were true | Sampling distribution of $t$ if H0 were true | Sampling distribution of $t$ if H0 were true | Sampling distribution of $t$ if H0 were true | Sampling distribution of $z$ if H0 were true | |
For main and interaction effects together (model):
| $t$ distribution with $n_1 + n_2 - 2$ degrees of freedom | Approximately the $t$ distribution with $N - 2$ degrees of freedom | $t$ distribution with $N - 1$ degrees of freedom | Standard normal distribution | |
Significant? | Significant? | Significant? | Significant? | Significant? | |
| Two sided:
| Two sided:
| Two sided:
| Two sided:
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n.a. | $C\%$ confidence interval for $\mu_1 - \mu_2$ | n.a. | $C\%$ confidence interval for $\mu$ | $C\%$ confidence interval for $\mu_1 - \mu_2$ | |
- | $(\bar{y}_1 - \bar{y}_2) \pm t^* \times s_p\sqrt{\dfrac{1}{n_1} + \dfrac{1}{n_2}}$
where the critical value $t^*$ is the value under the $t_{n_1 + n_2 - 2}$ distribution with the area $C / 100$ between $-t^*$ and $t^*$ (e.g. $t^*$ = 2.086 for a 95% confidence interval when df = 20). The confidence interval for $\mu_1 - \mu_2$ can also be used as significance test. | - | $\bar{y} \pm t^* \times \dfrac{s}{\sqrt{N}}$
where the critical value $t^*$ is the value under the $t_{N-1}$ distribution with the area $C / 100$ between $-t^*$ and $t^*$ (e.g. $t^*$ = 2.086 for a 95% confidence interval when df = 20). The confidence interval for $\mu$ can also be used as significance test. | $(\bar{y}_1 - \bar{y}_2) \pm z^* \times \sqrt{\dfrac{\sigma^2_1}{n_1} + \dfrac{\sigma^2_2}{n_2}}$
where the critical value $z^*$ is the value under the normal curve with the area $C / 100$ between $-z^*$ and $z^*$ (e.g. $z^*$ = 1.96 for a 95% confidence interval). The confidence interval for $\mu_1 - \mu_2$ can also be used as significance test. | |
Effect size | Effect size | n.a. | Effect size | n.a. | |
| Cohen's $d$: Standardized difference between the mean in group $1$ and in group $2$: $$d = \frac{\bar{y}_1 - \bar{y}_2}{s_p}$$ Cohen's $d$ indicates how many standard deviations $s_p$ the two sample means are removed from each other. | - | Cohen's $d$: Standardized difference between the sample mean of the difference scores and $\mu_0$: $$d = \frac{\bar{y} - \mu_0}{s}$$ Cohen's $d$ indicates how many standard deviations $s$ the sample mean of the difference scores $\bar{y}$ is removed from $\mu_0.$ | - | |
n.a. | Visual representation | n.a. | Visual representation | Visual representation | |
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ANOVA table | n.a. | n.a. | n.a. | n.a. | |
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Equivalent to | Equivalent to | n.a. | Equivalent to | n.a. | |
OLS regression with two categorical independent variables and the interaction term, transformed into $(I - 1)$ + $(J - 1)$ + $(I - 1) \times (J - 1)$ code variables. | One way ANOVA with an independent variable with 2 levels ($I$ = 2):
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Example context | Example context | Example context | Example context | Example context | |
Is the average mental health score different between people from a low, moderate, and high economic class? And is the average mental health score different between men and women? And is there an interaction effect between economic class and gender? | Is the average mental health score different between men and women? Assume that in the population, the standard deviation of mental health scores is equal amongst men and women. | Is there a monotonic relationship between physical health and mental health? | Is the average difference between the mental health scores before and after an intervention different from $\mu_0 = 0$? | Is the average mental health score different between men and women? Assume that in the population, the standard devation of the mental health scores is $\sigma_1 = 2$ amongst men and $\sigma_2 = 2.5$ amongst women. | |
SPSS | SPSS | SPSS | SPSS | n.a. | |
Analyze > General Linear Model > Univariate...
| Analyze > Compare Means > Independent-Samples T Test...
| Analyze > Correlate > Bivariate...
| Analyze > Compare Means > Paired-Samples T Test...
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Jamovi | Jamovi | Jamovi | Jamovi | n.a. | |
ANOVA > ANOVA
| T-Tests > Independent Samples T-Test
| Regression > Correlation Matrix
| T-Tests > Paired Samples T-Test
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Practice questions | Practice questions | Practice questions | Practice questions | Practice questions | |