Two way ANOVA - overview

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Two way ANOVA
McNemar's test
Goodness of fit test
Cochran's Q test
Independent/grouping variablesIndependent variableIndependent variableIndependent/grouping variable
Two categorical, the first with $I$ independent groups and the second with $J$ independent groups ($I \geqslant 2$, $J \geqslant 2$)2 paired groupsNoneOne within subject factor ($\geq 2$ related groups)
Dependent variableDependent variableDependent variableDependent variable
One quantitative of interval or ratio levelOne categorical with 2 independent groupsOne categorical with $J$ independent groups ($J \geqslant 2$)One categorical with 2 independent groups
Null hypothesisNull hypothesisNull hypothesisNull hypothesis
ANOVA $F$ tests:
  • H0 for main and interaction effects together (model): no main effects and interaction effect
  • H0 for independent variable A: no main effect for A
  • H0 for independent variable B: no main effect for B
  • H0 for the interaction term: no interaction effect between A and B
Like in one way ANOVA, we can also perform $t$ tests for specific contrasts and multiple comparisons. This is more advanced stuff.

Let's say that the scores on the dependent variable are scored 0 and 1. Then for each pair of scores, the data allow four options:

  1. First score of pair is 0, second score of pair is 0
  2. First score of pair is 0, second score of pair is 1 (switched)
  3. First score of pair is 1, second score of pair is 0 (switched)
  4. First score of pair is 1, second score of pair is 1
The null hypothesis H0 is that for each pair of scores, P(first score of pair is 0 while second score of pair is 1) = P(first score of pair is 1 while second score of pair is 0). That is, the probability that a pair of scores switches from 0 to 1 is the same as the probability that a pair of scores switches from 1 to 0.

Other formulations of the null hypothesis are:

  • H0: $\pi_1 = \pi_2$, where $\pi_1$ is the population proportion of ones for the first paired group and $\pi_2$ is the population proportion of ones for the second paired group
  • H0: for each pair of scores, P(first score of pair is 1) = P(second score of pair is 1)

  • H0: the population proportions in each of the $J$ conditions are $\pi_1$, $\pi_2$, $\ldots$, $\pi_J$
or equivalently
  • H0: the probability of drawing an observation from condition 1 is $\pi_1$, the probability of drawing an observation from condition 2 is $\pi_2$, $\ldots$, the probability of drawing an observation from condition $J$ is $\pi_J$
H0: $\pi_1 = \pi_2 = \ldots = \pi_I$

Here $\pi_1$ is the population proportion of 'successes' for group 1, $\pi_2$ is the population proportion of 'successes' for group 2, and $\pi_I$ is the population proportion of 'successes' for group $I.$
Alternative hypothesisAlternative hypothesisAlternative hypothesisAlternative hypothesis
ANOVA $F$ tests:
  • H1 for main and interaction effects together (model): there is a main effect for A, and/or for B, and/or an interaction effect
  • H1 for independent variable A: there is a main effect for A
  • H1 for independent variable B: there is a main effect for B
  • H1 for the interaction term: there is an interaction effect between A and B

The alternative hypothesis H1 is that for each pair of scores, P(first score of pair is 0 while second score of pair is 1) $\neq$ P(first score of pair is 1 while second score of pair is 0). That is, the probability that a pair of scores switches from 0 to 1 is not the same as the probability that a pair of scores switches from 1 to 0.

Other formulations of the alternative hypothesis are:

  • H1: $\pi_1 \neq \pi_2$
  • H1: for each pair of scores, P(first score of pair is 1) $\neq$ P(second score of pair is 1)

  • H1: the population proportions are not all as specified under the null hypothesis
or equivalently
  • H1: the probabilities of drawing an observation from each of the conditions are not all as specified under the null hypothesis
H1: not all population proportions are equal
AssumptionsAssumptionsAssumptionsAssumptions
  • Within each of the $I \times J$ populations, the scores on the dependent variable are normally distributed
  • The standard deviation of the scores on the dependent variable is the same in each of the $I \times J$ populations
  • For each of the $I \times J$ groups, the sample is an independent and simple random sample from the population defined by that group. That is, within and between groups, observations are independent of one another
  • Equal sample sizes for each group make the interpretation of the ANOVA output easier (unequal sample sizes result in overlap in the sum of squares; this is advanced stuff)
  • Sample of pairs is a simple random sample from the population of pairs. That is, pairs are independent of one another
  • Sample size is large enough for $X^2$ to be approximately chi-squared distributed. Rule of thumb: all $J$ expected cell counts are 5 or more
  • Sample is a simple random sample from the population. That is, observations are independent of one another
  • Sample of 'blocks' (usually the subjects) is a simple random sample from the population. That is, blocks are independent of one another
Test statisticTest statisticTest statisticTest statistic
For main and interaction effects together (model):
  • $F = \dfrac{\mbox{mean square model}}{\mbox{mean square error}}$
For independent variable A:
  • $F = \dfrac{\mbox{mean square A}}{\mbox{mean square error}}$
For independent variable B:
  • $F = \dfrac{\mbox{mean square B}}{\mbox{mean square error}}$
For the interaction term:
  • $F = \dfrac{\mbox{mean square interaction}}{\mbox{mean square error}}$
Note: mean square error is also known as mean square residual or mean square within.
$X^2 = \dfrac{(b - c)^2}{b + c}$
Here $b$ is the number of pairs in the sample for which the first score is 0 while the second score is 1, and $c$ is the number of pairs in the sample for which the first score is 1 while the second score is 0.
$X^2 = \sum{\frac{(\mbox{observed cell count} - \mbox{expected cell count})^2}{\mbox{expected cell count}}}$
Here the expected cell count for one cell = $N \times \pi_j$, the observed cell count is the observed sample count in that same cell, and the sum is over all $J$ cells.
If a failure is scored as 0 and a success is scored as 1:

$Q = k(k - 1) \dfrac{\sum_{groups} \Big (\mbox{group total} - \frac{\mbox{grand total}}{k} \Big)^2}{\sum_{blocks} \mbox{block total} \times (k - \mbox{block total})}$

Here $k$ is the number of related groups (usually the number of repeated measurements), a group total is the sum of the scores in a group, a block total is the sum of the scores in a block (usually a subject), and the grand total is the sum of all the scores.

Before computing $Q$, first exclude blocks with equal scores in all $k$ groups.
Pooled standard deviationn.a.n.a.n.a.
$ \begin{aligned} s_p &= \sqrt{\dfrac{\sum\nolimits_{subjects} (\mbox{subject's score} - \mbox{its group mean})^2}{N - (I \times J)}}\\ &= \sqrt{\dfrac{\mbox{sum of squares error}}{\mbox{degrees of freedom error}}}\\ &= \sqrt{\mbox{mean square error}} \end{aligned} $ ---
Sampling distribution of $F$ if H0 were trueSampling distribution of $X^2$ if H0 were trueSampling distribution of $X^2$ if H0 were trueSampling distribution of $Q$ if H0 were true
For main and interaction effects together (model):
  • $F$ distribution with $(I - 1) + (J - 1) + (I - 1) \times (J - 1)$ (df model, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
For independent variable A:
  • $F$ distribution with $I - 1$ (df A, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
For independent variable B:
  • $F$ distribution with $J - 1$ (df B, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
For the interaction term:
  • $F$ distribution with $(I - 1) \times (J - 1)$ (df interaction, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
Here $N$ is the total sample size.

If $b + c$ is large enough (say, > 20), approximately the chi-squared distribution with 1 degree of freedom.

If $b + c$ is small, the Binomial($n$, $P$) distribution should be used, with $n = b + c$ and $P = 0.5$. In that case the test statistic becomes equal to $b$.

Approximately the chi-squared distribution with $J - 1$ degrees of freedomIf the number of blocks (usually the number of subjects) is large, approximately the chi-squared distribution with $k - 1$ degrees of freedom
Significant?Significant?Significant?Significant?
  • Check if $F$ observed in sample is equal to or larger than critical value $F^*$ or
  • Find $p$ value corresponding to observed $F$ and check if it is equal to or smaller than $\alpha$
For test statistic $X^2$:
  • Check if $X^2$ observed in sample is equal to or larger than critical value $X^{2*}$ or
  • Find $p$ value corresponding to observed $X^2$ and check if it is equal to or smaller than $\alpha$
If $b + c$ is small, the table for the binomial distribution should be used, with as test statistic $b$:
  • Check if $b$ observed in sample is in the rejection region or
  • Find two sided $p$ value corresponding to observed $b$ and check if it is equal to or smaller than $\alpha$
  • Check if $X^2$ observed in sample is equal to or larger than critical value $X^{2*}$ or
  • Find $p$ value corresponding to observed $X^2$ and check if it is equal to or smaller than $\alpha$
If the number of blocks is large, the table with critical $X^2$ values can be used. If we denote $X^2 = Q$:
  • Check if $X^2$ observed in sample is equal to or larger than critical value $X^{2*}$ or
  • Find $p$ value corresponding to observed $X^2$ and check if it is equal to or smaller than $\alpha$
Effect sizen.a.n.a.n.a.
  • Proportion variance explained $R^2$:
    Proportion variance of the dependent variable $y$ explained by the independent variables and the interaction effect together:
    $$ \begin{align} R^2 &= \dfrac{\mbox{sum of squares model}}{\mbox{sum of squares total}} \end{align} $$ $R^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population.

  • Proportion variance explained $\eta^2$:
    Proportion variance of the dependent variable $y$ explained by an independent variable or interaction effect:
    $$ \begin{align} \eta^2_A &= \dfrac{\mbox{sum of squares A}}{\mbox{sum of squares total}}\\ \\ \eta^2_B &= \dfrac{\mbox{sum of squares B}}{\mbox{sum of squares total}}\\ \\ \eta^2_{int} &= \dfrac{\mbox{sum of squares int}}{\mbox{sum of squares total}} \end{align} $$ $\eta^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population.

  • Proportion variance explained $\omega^2$:
    Corrects for the positive bias in $\eta^2$ and is equal to:
    $$ \begin{align} \omega^2_A &= \dfrac{\mbox{sum of squares A} - \mbox{degrees of freedom A} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \\ \omega^2_B &= \dfrac{\mbox{sum of squares B} - \mbox{degrees of freedom B} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \\ \omega^2_{int} &= \dfrac{\mbox{sum of squares int} - \mbox{degrees of freedom int} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \end{align} $$ $\omega^2$ is a better estimate of the explained variance in the population than $\eta^2$. Only for balanced designs (equal sample sizes).

  • Proportion variance explained $\eta^2_{partial}$: $$ \begin{align} \eta^2_{partial\,A} &= \frac{\mbox{sum of squares A}}{\mbox{sum of squares A} + \mbox{sum of squares error}}\\ \\ \eta^2_{partial\,B} &= \frac{\mbox{sum of squares B}}{\mbox{sum of squares B} + \mbox{sum of squares error}}\\ \\ \eta^2_{partial\,int} &= \frac{\mbox{sum of squares int}}{\mbox{sum of squares int} + \mbox{sum of squares error}} \end{align} $$
---
ANOVA tablen.a.n.a.n.a.
two way ANOVA table
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Equivalent toEquivalent ton.a.Equivalent to
OLS regression with two categorical independent variables and the interaction term, transformed into $(I - 1)$ + $(J - 1)$ + $(I - 1) \times (J - 1)$ code variables.-Friedman test, with a categorical dependent variable consisting of two independent groups.
Example contextExample contextExample contextExample context
Is the average mental health score different between people from a low, moderate, and high economic class? And is the average mental health score different between men and women? And is there an interaction effect between economic class and gender?Does a tv documentary about spiders change whether people are afraid (yes/no) of spiders?Is the proportion of people with a low, moderate, and high social economic status in the population different from $\pi_{low} = 0.2,$ $\pi_{moderate} = 0.6,$ and $\pi_{high} = 0.2$?Subjects perform three different tasks, which they can either perform correctly or incorrectly. Is there a difference in task performance between the three different tasks?
SPSSSPSSSPSSSPSS
Analyze > General Linear Model > Univariate...
  • Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factor(s)
Analyze > Nonparametric Tests > Legacy Dialogs > 2 Related Samples...
  • Put the two paired variables in the boxes below Variable 1 and Variable 2
  • Under Test Type, select the McNemar test
Analyze > Nonparametric Tests > Legacy Dialogs > Chi-square...
  • Put your categorical variable in the box below Test Variable List
  • Fill in the population proportions / probabilities according to $H_0$ in the box below Expected Values. If $H_0$ states that they are all equal, just pick 'All categories equal' (default)
Analyze > Nonparametric Tests > Legacy Dialogs > K Related Samples...
  • Put the $k$ variables containing the scores for the $k$ related groups in the white box below Test Variables
  • Under Test Type, select Cochran's Q test
JamoviJamoviJamoviJamovi
ANOVA > ANOVA
  • Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factors
Frequencies > Paired Samples - McNemar test
  • Put one of the two paired variables in the box below Rows and the other paired variable in the box below Columns
Frequencies > N Outcomes - $\chi^2$ Goodness of fit
  • Put your categorical variable in the box below Variable
  • Click on Expected Proportions and fill in the population proportions / probabilities according to $H_0$ in the boxes below Ratio. If $H_0$ states that they are all equal, you can leave the ratios equal to the default values (1)
Jamovi does not have a specific option for the Cochran's Q test. However, you can do the Friedman test instead. The $p$ value resulting from this Friedman test is equivalent to the $p$ value that would have resulted from the Cochran's Q test. Go to:

ANOVA > Repeated Measures ANOVA - Friedman
  • Put the $k$ variables containing the scores for the $k$ related groups in the box below Measures
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