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Two way ANOVA
Chisquared test for the relationship between two categorical variables
Two categorical, the first with $I$ independent groups and the second with $J$ independent groups ($I \geqslant 2$, $J \geqslant 2$)
One categorical with $I$ independent groups ($I \geqslant 2$)
None
One within subject factor ($\geq 2$ related groups)
One categorical with 2 independent groups
One categorical with 2 independent groups
Dependent variable
Dependent /row variable
Dependent variable
Dependent variable
Dependent variable
Dependent variable
One quantitative of interval or ratio level
One categorical with $J$ independent groups ($J \geqslant 2$)
One quantitative of interval or ratio level
One of ordinal level
One of ordinal level
One of ordinal level
Null hypothesis
Null hypothesis
Null hypothesis
Null hypothesis
Null hypothesis
Null hypothesis
ANOVA $F$ tests:
H_{0} for main and interaction effects together (model): no main effects and interaction effect
H_{0} for independent variable A: no main effect for A
H_{0} for independent variable B: no main effect for B
H_{0} for the interaction term: no interaction effect between A and B
Like in one way ANOVA, we can also perform $t$ tests for specific contrasts and multiple comparisons. This is more advanced stuff.
H_{0}: there is no association between the row and column variable
More precisely, if there are $I$ independent random samples of size $n_i$ from each of $I$ populations, defined by the independent variable:
H_{0}: the distribution of the dependent variable is the same in each of the $I$ populations
If there is one random sample of size $N$ from the total population:
H_{0}: the row and column variables are independent
H_{0}: $\mu = \mu_0$
Here $\mu$ is the population mean, and $\mu_0$ is the population mean according to the null hypothesis.
H_{0}: the population scores in any of the related groups are not systematically higher or lower than the population scores in any of the other related groups
Usually the related groups are the different measurement points. Several different formulations of the null hypothesis can be found in the literature, and we do not agree with all of them. Make sure you (also) learn the one that is given in your text book or by your teacher.
If the dependent variable is measured on a continuous scale and the shape of the distribution of the dependent variable is the same in both populations:
H_{0}: the population median for group 1 is equal to the population median for group 2
Else:
Formulation 1:
H_{0}: the population scores in group 1 are not systematically higher or lower than the population scores in group 2
Formulation 2:
H_{0}:
P(an observation from population 1 exceeds an observation from population 2) = P(an observation from population 2 exceeds observation from population 1)
Several different formulations of the null hypothesis can be found in the literature, and we do not agree with all of them. Make sure you (also) learn the one that is given in your text book or by your teacher.
If the dependent variable is measured on a continuous scale and the shape of the distribution of the dependent variable is the same in both populations:
H_{0}: the population median for group 1 is equal to the population median for group 2
Else:
Formulation 1:
H_{0}: the population scores in group 1 are not systematically higher or lower than the population scores in group 2
Formulation 2:
H_{0}:
P(an observation from population 1 exceeds an observation from population 2) = P(an observation from population 2 exceeds observation from population 1)
Several different formulations of the null hypothesis can be found in the literature, and we do not agree with all of them. Make sure you (also) learn the one that is given in your text book or by your teacher.
Alternative hypothesis
Alternative hypothesis
Alternative hypothesis
Alternative hypothesis
Alternative hypothesis
Alternative hypothesis
ANOVA $F$ tests:
H_{1} for main and interaction effects together (model): there is a main effect for A, and/or for B, and/or an interaction effect
H_{1} for independent variable A: there is a main effect for A
H_{1} for independent variable B: there is a main effect for B
H_{1} for the interaction term: there is an interaction effect between A and B
H_{1}: there is an association between the row and column variable
More precisely, if there are $I$ independent random samples of size $n_i$ from each of $I$ populations, defined by the independent variable:
H_{1}: the distribution of the dependent variable is not the same in all of the $I$ populations
If there is one random sample of size $N$ from the total population:
H_{1}: the row and column variables are dependent
H_{1} two sided: $\mu \neq \mu_0$
H_{1} right sided: $\mu > \mu_0$
H_{1} left sided: $\mu < \mu_0$
H_{1}: the population scores in some of the related groups are systematically higher or lower than the population scores in other related groups
If the dependent variable is measured on a continuous scale and the shape of the distribution of the dependent variable is the same in both populations:
H_{1} two sided: the population median for group 1 is not equal to the population median for group 2
H_{1} right sided: the population median for group 1 is larger than the population median for group 2
H_{1} left sided: the population median for group 1 is smaller than the population median for group 2
Else:
Formulation 1:
H_{1} two sided: the population scores in group 1 are systematically higher or lower than the population scores in group 2
H_{1} right sided: the population scores in group 1 are systematically higher than the population scores in group 2
H_{1} left sided: the population scores in group 1 are systematically lower than the population scores in group 2
Formulation 2:
H_{1} two sided: P(an observation from population 1 exceeds an observation from population 2) $\neq$ P(an observation from population 2 exceeds an observation from population 1)
H_{1} right sided: P(an observation from population 1 exceeds an observation from population 2) > P(an observation from population 2 exceeds an observation from population 1)
H_{1} left sided: P(an observation from population 1 exceeds an observation from population 2) < P(an observation from population 2 exceeds an observation from population 1)
If the dependent variable is measured on a continuous scale and the shape of the distribution of the dependent variable is the same in both populations:
H_{1} two sided: the population median for group 1 is not equal to the population median for group 2
H_{1} right sided: the population median for group 1 is larger than the population median for group 2
H_{1} left sided: the population median for group 1 is smaller than the population median for group 2
Else:
Formulation 1:
H_{1} two sided: the population scores in group 1 are systematically higher or lower than the population scores in group 2
H_{1} right sided: the population scores in group 1 are systematically higher than the population scores in group 2
H_{1} left sided: the population scores in group 1 are systematically lower than the population scores in group 2
Formulation 2:
H_{1} two sided: P(an observation from population 1 exceeds an observation from population 2) $\neq$ P(an observation from population 2 exceeds an observation from population 1)
H_{1} right sided: P(an observation from population 1 exceeds an observation from population 2) > P(an observation from population 2 exceeds an observation from population 1)
H_{1} left sided: P(an observation from population 1 exceeds an observation from population 2) < P(an observation from population 2 exceeds an observation from population 1)
Assumptions
Assumptions
Assumptions
Assumptions
Assumptions
Assumptions
Within each of the $I \times J$ populations, the scores on the dependent variable are normally distributed
The standard deviation of the scores on the dependent variable is the same in each of the $I \times J$ populations
For each of the $I \times J$ groups, the sample is an independent and simple random sample from the population defined by that group. That is, within and between groups, observations are independent of one another
Equal sample sizes for each group make the interpretation of the ANOVA output easier (unequal sample sizes result in overlap in the sum of squares; this is advanced stuff)
Sample size is large enough for $X^2$ to be approximately chisquared distributed under the null hypothesis. Rule of thumb:
2 $\times$ 2 table: all four expected cell counts are 5 or more
Larger than 2 $\times$ 2 tables: average of the expected cell counts is 5 or more, smallest expected cell count is 1 or more
There are $I$ independent simple random samples from each of $I$ populations defined by the independent variable, or there is one simple random sample from the total population
Scores are normally distributed in the population
Population standard deviation $\sigma$ is known
Sample is a simple random sample from the population. That is, observations are independent of one another
Sample of 'blocks' (usually the subjects) is a simple random sample from the population. That is, blocks are independent of one another
Group 1 sample is a simple random sample (SRS) from population 1, group 2 sample is an independent SRS from population 2. That is, within and between groups, observations are independent of one another
Group 1 sample is a simple random sample (SRS) from population 1, group 2 sample is an independent SRS from population 2. That is, within and between groups, observations are independent of one another
Test statistic
Test statistic
Test statistic
Test statistic
Test statistic
Test statistic
For main and interaction effects together (model):
Note: mean square error is also known as mean square residual or mean square within.
$X^2 = \sum{\frac{(\mbox{observed cell count}  \mbox{expected cell count})^2}{\mbox{expected cell count}}}$
Here for each cell, the expected cell count = $\dfrac{\mbox{row total} \times \mbox{column total}}{\mbox{total sample size}}$, the observed cell count is the observed sample count in that same cell, and the sum is over all $I \times J$ cells.
$z = \dfrac{\bar{y}  \mu_0}{\sigma / \sqrt{N}}$
Here $\bar{y}$ is the sample mean, $\mu_0$ is the population mean according to the null hypothesis, $\sigma$ is the population standard deviation, and $N$ is the sample size.
Here $N$ is the number of 'blocks' (usually the subjects  so if you have 4 repeated measurements for 60 subjects, $N$ equals 60), $k$ is the number of related groups (usually the number of repeated measurements), and $R_i$ is the sum of ranks in group $i$.
Remember that multiplication precedes addition, so first compute $\frac{12}{N \times k(k + 1)} \times \sum R^2_i$ and then subtract $3 \times N(k + 1)$.
Note: if ties are present in the data, the formula for $Q$ is more complicated.
Two different types of test statistics can be used; both will result in the same test outcome. The first is the Wilcoxon rank sum statistic $W$:
The second type of test statistic is the MannWhitney $U$ statistic:
$U = W  \dfrac{n_1(n_1 + 1)}{2}$
where $n_1$ is the sample size of group 1.
Note: we could just as well base W and U on group 2. This would only 'flip' the right and left sided alternative hypotheses. Also, tables with critical values for $U$ are often based on the smaller of $U$ for group 1 and for group 2.
Two different types of test statistics can be used; both will result in the same test outcome. The first is the Wilcoxon rank sum statistic $W$:
The second type of test statistic is the MannWhitney $U$ statistic:
$U = W  \dfrac{n_1(n_1 + 1)}{2}$
where $n_1$ is the sample size of group 1.
Note: we could just as well base W and U on group 2. This would only 'flip' the right and left sided alternative hypotheses. Also, tables with critical values for $U$ are often based on the smaller of $U$ for group 1 and for group 2.
Pooled standard deviation
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$
\begin{aligned}
s_p &= \sqrt{\dfrac{\sum\nolimits_{subjects} (\mbox{subject's score}  \mbox{its group mean})^2}{N  (I \times J)}}\\
&= \sqrt{\dfrac{\mbox{sum of squares error}}{\mbox{degrees of freedom error}}}\\
&= \sqrt{\mbox{mean square error}}
\end{aligned}
$
Sampling distribution of $W$ and of $U$ if H_{0} were true
Sampling distribution of $W$ and of $U$ if H_{0} were true
For main and interaction effects together (model):
$F$ distribution with $(I  1) + (J  1) + (I  1) \times (J  1)$ (df model, numerator) and $N  (I \times J)$ (df error, denominator) degrees of freedom
For independent variable A:
$F$ distribution with $I  1$ (df A, numerator) and $N  (I \times J)$ (df error, denominator) degrees of freedom
For independent variable B:
$F$ distribution with $J  1$ (df B, numerator) and $N  (I \times J)$ (df error, denominator) degrees of freedom
For the interaction term:
$F$ distribution with $(I  1) \times (J  1)$ (df interaction, numerator) and $N  (I \times J)$ (df error, denominator) degrees of freedom
Here $N$ is the total sample size.
Approximately the chisquared distribution with $(I  1) \times (J  1)$ degrees of freedom
Standard normal distribution
If the number of blocks $N$ is large, approximately the chisquared distribution with $k  1$ degrees of freedom.
For small samples, the exact distribution of
$Q$ should be used.
Sampling distribution of $W$:
For large samples, $W$ is approximately normally distributed with mean $\mu_W$ and standard deviation $\sigma_W$ if the null hypothesis were true. Here
$$
\begin{aligned}
\mu_W &= \dfrac{n_1(n_1 + n_2 + 1)}{2}\\
\sigma_W &= \sqrt{\dfrac{n_1 n_2(n_1 + n_2 + 1)}{12}}
\end{aligned}
$$
Hence, for large samples, the standardized test statistic
$$
z_W = \dfrac{W  \mu_W}{\sigma_W}\\
$$
follows approximately the standard normal distribution if the null hypothesis were true. Note that if your $W$ value is based on group 2, $\mu_W$ becomes $\frac{n_2(n_1 + n_2 + 1)}{2}$.
Sampling distribution of $U$:
For large samples, $U$ is approximately normally distributed with mean $\mu_U$ and standard deviation $\sigma_U$ if the null hypothesis were true. Here
$$
\begin{aligned}
\mu_U &= \dfrac{n_1 n_2}{2}\\
\sigma_U &= \sqrt{\dfrac{n_1 n_2(n_1 + n_2 + 1)}{12}}
\end{aligned}
$$
Hence, for large samples, the standardized test statistic
$$
z_U = \dfrac{U  \mu_U}{\sigma_U}\\
$$
follows approximately the standard normal distribution if the null hypothesis were true.
For small samples, the exact distribution of $W$ or $U$ should be used.
Note: if ties are present in the data, the formula for the standard deviations $\sigma_W$ and $\sigma_U$ is more complicated.
Sampling distribution of $W$:
For large samples, $W$ is approximately normally distributed with mean $\mu_W$ and standard deviation $\sigma_W$ if the null hypothesis were true. Here
$$
\begin{aligned}
\mu_W &= \dfrac{n_1(n_1 + n_2 + 1)}{2}\\
\sigma_W &= \sqrt{\dfrac{n_1 n_2(n_1 + n_2 + 1)}{12}}
\end{aligned}
$$
Hence, for large samples, the standardized test statistic
$$
z_W = \dfrac{W  \mu_W}{\sigma_W}\\
$$
follows approximately the standard normal distribution if the null hypothesis were true. Note that if your $W$ value is based on group 2, $\mu_W$ becomes $\frac{n_2(n_1 + n_2 + 1)}{2}$.
Sampling distribution of $U$:
For large samples, $U$ is approximately normally distributed with mean $\mu_U$ and standard deviation $\sigma_U$ if the null hypothesis were true. Here
$$
\begin{aligned}
\mu_U &= \dfrac{n_1 n_2}{2}\\
\sigma_U &= \sqrt{\dfrac{n_1 n_2(n_1 + n_2 + 1)}{12}}
\end{aligned}
$$
Hence, for large samples, the standardized test statistic
$$
z_U = \dfrac{U  \mu_U}{\sigma_U}\\
$$
follows approximately the standard normal distribution if the null hypothesis were true.
For small samples, the exact distribution of $W$ or $U$ should be used.
Note: if ties are present in the data, the formula for the standard deviations $\sigma_W$ and $\sigma_U$ is more complicated.
Significant?
Significant?
Significant?
Significant?
Significant?
Significant?
Check if $F$ observed in sample is equal to or larger than critical value $F^*$ or
Find $p$ value corresponding to observed $F$ and check if it is equal to or smaller than $\alpha$
Find $p$ value corresponding to observed $X^2$ and check if it is equal to or smaller than $\alpha$
For large samples, the table for standard normal probabilities can be used:
Two sided:
Check if $z$ observed in sample is at least as extreme as critical value $z^*$ or
Find two sided $p$ value corresponding to observed $z$ and check if it is equal to or smaller than $\alpha$
Right sided:
Check if $z$ observed in sample is equal to or larger than critical value $z^*$ or
Find right sided $p$ value corresponding to observed $z$ and check if it is equal to or smaller than $\alpha$
Left sided:
Check if $z$ observed in sample is equal to or smaller than critical value $z^*$ or
Find left sided $p$ value corresponding to observed $z$ and check if it is equal to or smaller than $\alpha$
For large samples, the table for standard normal probabilities can be used:
Two sided:
Check if $z$ observed in sample is at least as extreme as critical value $z^*$ or
Find two sided $p$ value corresponding to observed $z$ and check if it is equal to or smaller than $\alpha$
Right sided:
Check if $z$ observed in sample is equal to or larger than critical value $z^*$ or
Find right sided $p$ value corresponding to observed $z$ and check if it is equal to or smaller than $\alpha$
Left sided:
Check if $z$ observed in sample is equal to or smaller than critical value $z^*$ or
Find left sided $p$ value corresponding to observed $z$ and check if it is equal to or smaller than $\alpha$
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$C\%$ confidence interval for $\mu$
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$\bar{y} \pm z^* \times \dfrac{\sigma}{\sqrt{N}}$
where the critical value $z^*$ is the value under the normal curve with the area $C / 100$ between $z^*$ and $z^*$ (e.g. $z^*$ = 1.96 for a 95% confidence interval).
Proportion variance explained $R^2$:
Proportion variance of the dependent variable $y$ explained by the independent variables and the interaction effect together:
$$
\begin{align}
R^2 &= \dfrac{\mbox{sum of squares model}}{\mbox{sum of squares total}}
\end{align}
$$
$R^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population.
Proportion variance explained $\eta^2$:
Proportion variance of the dependent variable $y$ explained by an independent variable or interaction effect:
$$
\begin{align}
\eta^2_A &= \dfrac{\mbox{sum of squares A}}{\mbox{sum of squares total}}\\
\\
\eta^2_B &= \dfrac{\mbox{sum of squares B}}{\mbox{sum of squares total}}\\
\\
\eta^2_{int} &= \dfrac{\mbox{sum of squares int}}{\mbox{sum of squares total}}
\end{align}
$$
$\eta^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population.
Proportion variance explained $\omega^2$:
Corrects for the positive bias in $\eta^2$ and is equal to:
$$
\begin{align}
\omega^2_A &= \dfrac{\mbox{sum of squares A}  \mbox{degrees of freedom A} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\
\\
\omega^2_B &= \dfrac{\mbox{sum of squares B}  \mbox{degrees of freedom B} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\
\\
\omega^2_{int} &= \dfrac{\mbox{sum of squares int}  \mbox{degrees of freedom int} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\
\end{align}
$$
$\omega^2$ is a better estimate of the explained variance in the population than
$\eta^2$. Only for balanced designs (equal sample sizes).
Proportion variance explained $\eta^2_{partial}$:
$$
\begin{align}
\eta^2_{partial\,A} &= \frac{\mbox{sum of squares A}}{\mbox{sum of squares A} + \mbox{sum of squares error}}\\
\\
\eta^2_{partial\,B} &= \frac{\mbox{sum of squares B}}{\mbox{sum of squares B} + \mbox{sum of squares error}}\\
\\
\eta^2_{partial\,int} &= \frac{\mbox{sum of squares int}}{\mbox{sum of squares int} + \mbox{sum of squares error}}
\end{align}
$$

Cohen's $d$:
Standardized difference between the sample mean and $\mu_0$:
$$d = \frac{\bar{y}  \mu_0}{\sigma}$$
Cohen's $d$ indicates how many standard deviations $\sigma$ the sample mean $\bar{y}$ is removed from $\mu_0.$



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Visual representation
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ANOVA table
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Equivalent to
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Equivalent to
Equivalent to
OLS regression with two categorical independent variables and the interaction term, transformed into $(I  1)$ + $(J  1)$ + $(I  1) \times (J  1)$ code variables.



If there are no ties in the data, the two sided MannWhitneyWilcoxon test is equivalent to the KruskalWallis test with an independent variable with 2 levels ($I$ = 2).
If there are no ties in the data, the two sided MannWhitneyWilcoxon test is equivalent to the KruskalWallis test with an independent variable with 2 levels ($I$ = 2).
Example context
Example context
Example context
Example context
Example context
Example context
Is the average mental health score different between people from a low, moderate, and high economic class? And is the average mental health score different between men and women? And is there an interaction effect between economic class and gender?
Is there an association between economic class and gender? Is the distribution of economic class different between men and women?
Is the average mental health score of office workers different from $\mu_0 = 50$? Assume that the standard deviation of the mental health scores in the population is $\sigma = 3.$
Is there a difference in depression level between measurement point 1 (preintervention), measurement point 2 (1 week postintervention), and measurement point 3 (6 weeks postintervention)?
Do men tend to score higher on social economic status than women?
Do men tend to score higher on social economic status than women?
SPSS
SPSS
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SPSS
SPSS
SPSS
Analyze > General Linear Model > Univariate...
Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factor(s)
Analyze > Descriptive Statistics > Crosstabs...
Put one of your two categorical variables in the box below Row(s), and the other categorical variable in the box below Column(s)
Click the Statistics... button, and click on the square in front of Chisquare
Continue and click OK

Analyze > Nonparametric Tests > Legacy Dialogs > K Related Samples...
Put the $k$ variables containing the scores for the $k$ related groups in the white box below Test Variables
Put your dependent variable in the box below Test Variable List and your independent (grouping) variable in the box below Grouping Variable
Click on the Define Groups... button. If you can't click on it, first click on the grouping variable so its background turns yellow
Fill in the value you have used to indicate your first group in the box next to Group 1, and the value you have used to indicate your second group in the box next to Group 2
Put your dependent variable in the box below Test Variable List and your independent (grouping) variable in the box below Grouping Variable
Click on the Define Groups... button. If you can't click on it, first click on the grouping variable so its background turns yellow
Fill in the value you have used to indicate your first group in the box next to Group 1, and the value you have used to indicate your second group in the box next to Group 2
Continue and click OK
Jamovi
Jamovi
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Jamovi
Jamovi
Jamovi
ANOVA > ANOVA
Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factors
Frequencies > Independent Samples  $\chi^2$ test of association
Put one of your two categorical variables in the box below Rows, and the other categorical variable in the box below Columns

ANOVA > Repeated Measures ANOVA  Friedman
Put the $k$ variables containing the scores for the $k$ related groups in the box below Measures
TTests > Independent Samples TTest
Put your dependent variable in the box below Dependent Variables and your independent (grouping) variable in the box below Grouping Variable
Under Tests, select MannWhitney U
Under Hypothesis, select your alternative hypothesis
TTests > Independent Samples TTest
Put your dependent variable in the box below Dependent Variables and your independent (grouping) variable in the box below Grouping Variable
Under Tests, select MannWhitney U
Under Hypothesis, select your alternative hypothesis