Two way ANOVA overview

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Two way ANOVA	Chi-squared test for the relationship between two categorical variables	One sample $z$ test for the mean	Cochran's Q test
Independent/grouping variables	Independent /column variable	Independent variable	Independent/grouping variable
Two categorical, the first with $I$ independent groups and the second with $J$ independent groups ($I \geqslant 2$, $J \geqslant 2$)	One categorical with $I$ independent groups ($I \geqslant 2$)	None	One within subject factor ($\geq 2$ related groups)
Dependent variable	Dependent /row variable	Dependent variable	Dependent variable
One quantitative of interval or ratio level	One categorical with $J$ independent groups ($J \geqslant 2$)	One quantitative of interval or ratio level	One categorical with 2 independent groups
Null hypothesis	Null hypothesis	Null hypothesis	Null hypothesis
ANOVA $F$ tests: H₀ for main and interaction effects together (model): no main effects and interaction effect H₀ for independent variable A: no main effect for A H₀ for independent variable B: no main effect for B H₀ for the interaction term: no interaction effect between A and B Like in one way ANOVA, we can also perform $t$ tests for specific contrasts and multiple comparisons. This is more advanced stuff.	H₀: there is no association between the row and column variable More precisely, if there are $I$ independent random samples of size $n_i$ from each of $I$ populations, defined by the independent variable: H₀: the distribution of the dependent variable is the same in each of the $I$ populations If there is one random sample of size $N$ from the total population: H₀: the row and column variables are independent	H₀: $\mu = \mu_0$ Here $\mu$ is the population mean, and $\mu_0$ is the population mean according to the null hypothesis.	H₀: $\pi_1 = \pi_2 = \ldots = \pi_I$ Here $\pi_1$ is the population proportion of 'successes' for group 1, $\pi_2$ is the population proportion of 'successes' for group 2, and $\pi_I$ is the population proportion of 'successes' for group $I.$
Alternative hypothesis	Alternative hypothesis	Alternative hypothesis	Alternative hypothesis
ANOVA $F$ tests: H₁ for main and interaction effects together (model): there is a main effect for A, and/or for B, and/or an interaction effect H₁ for independent variable A: there is a main effect for A H₁ for independent variable B: there is a main effect for B H₁ for the interaction term: there is an interaction effect between A and B	H₁: there is an association between the row and column variable More precisely, if there are $I$ independent random samples of size $n_i$ from each of $I$ populations, defined by the independent variable: H₁: the distribution of the dependent variable is not the same in all of the $I$ populations If there is one random sample of size $N$ from the total population: H₁: the row and column variables are dependent	H₁ two sided: $\mu \neq \mu_0$ H₁ right sided: $\mu > \mu_0$ H₁ left sided: $\mu < \mu_0$	H₁: not all population proportions are equal
Assumptions	Assumptions	Assumptions	Assumptions
Within each of the $I \times J$ populations, the scores on the dependent variable are normally distributed The standard deviation of the scores on the dependent variable is the same in each of the $I \times J$ populations For each of the $I \times J$ groups, the sample is an independent and simple random sample from the population defined by that group. That is, within and between groups, observations are independent of one another Equal sample sizes for each group make the interpretation of the ANOVA output easier (unequal sample sizes result in overlap in the sum of squares; this is advanced stuff)	Sample size is large enough for $X^2$ to be approximately chi-squared distributed under the null hypothesis. Rule of thumb: 2 $\times$ 2 table: all four expected cell counts are 5 or more Larger than 2 $\times$ 2 tables: average of the expected cell counts is 5 or more, smallest expected cell count is 1 or more There are $I$ independent simple random samples from each of $I$ populations defined by the independent variable, or there is one simple random sample from the total population	Scores are normally distributed in the population Population standard deviation $\sigma$ is known Sample is a simple random sample from the population. That is, observations are independent of one another	Sample of 'blocks' (usually the subjects) is a simple random sample from the population. That is, blocks are independent of one another
Test statistic	Test statistic	Test statistic	Test statistic
For main and interaction effects together (model): $F = \dfrac{\mbox{mean square model}}{\mbox{mean square error}}$ For independent variable A: $F = \dfrac{\mbox{mean square A}}{\mbox{mean square error}}$ For independent variable B: $F = \dfrac{\mbox{mean square B}}{\mbox{mean square error}}$ For the interaction term: $F = \dfrac{\mbox{mean square interaction}}{\mbox{mean square error}}$ Note: mean square error is also known as mean square residual or mean square within.	$X^2 = \sum{\frac{(\mbox{observed cell count} - \mbox{expected cell count})^2}{\mbox{expected cell count}}}$ Here for each cell, the expected cell count = $\dfrac{\mbox{row total} \times \mbox{column total}}{\mbox{total sample size}}$, the observed cell count is the observed sample count in that same cell, and the sum is over all $I \times J$ cells.	$z = \dfrac{\bar{y} - \mu_0}{\sigma / \sqrt{N}}$ Here $\bar{y}$ is the sample mean, $\mu_0$ is the population mean according to the null hypothesis, $\sigma$ is the population standard deviation, and $N$ is the sample size. The denominator $\sigma / \sqrt{N}$ is the standard deviation of the sampling distribution of $\bar{y}$. The $z$ value indicates how many of these standard deviations $\bar{y}$ is removed from $\mu_0$.	If a failure is scored as 0 and a success is scored as 1: $Q = k(k - 1) \dfrac{\sum_{groups} \Big (\mbox{group total} - \frac{\mbox{grand total}}{k} \Big)^2}{\sum_{blocks} \mbox{block total} \times (k - \mbox{block total})}$ Here $k$ is the number of related groups (usually the number of repeated measurements), a group total is the sum of the scores in a group, a block total is the sum of the scores in a block (usually a subject), and the grand total is the sum of all the scores. Before computing $Q$, first exclude blocks with equal scores in all $k$ groups.
Pooled standard deviation	n.a.	n.a.	n.a.
$ \begin{aligned} s_p &= \sqrt{\dfrac{\sum\nolimits_{subjects} (\mbox{subject's score} - \mbox{its group mean})^2}{N - (I \times J)}}\\ &= \sqrt{\dfrac{\mbox{sum of squares error}}{\mbox{degrees of freedom error}}}\\ &= \sqrt{\mbox{mean square error}} \end{aligned} $	-	-	-
Sampling distribution of $F$ if H₀ were true	Sampling distribution of $X^2$ if H₀ were true	Sampling distribution of $z$ if H₀ were true	Sampling distribution of $Q$ if H₀ were true
For main and interaction effects together (model): $F$ distribution with $(I - 1) + (J - 1) + (I - 1) \times (J - 1)$ (df model, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom For independent variable A: $F$ distribution with $I - 1$ (df A, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom For independent variable B: $F$ distribution with $J - 1$ (df B, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom For the interaction term: $F$ distribution with $(I - 1) \times (J - 1)$ (df interaction, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom Here $N$ is the total sample size.	Approximately the chi-squared distribution with $(I - 1) \times (J - 1)$ degrees of freedom	Standard normal distribution	If the number of blocks (usually the number of subjects) is large, approximately the chi-squared distribution with $k - 1$ degrees of freedom
Significant?	Significant?	Significant?	Significant?
Check if $F$ observed in sample is equal to or larger than critical value $F^*$ or Find $p$ value corresponding to observed $F$ and check if it is equal to or smaller than $\alpha$	Check if $X^2$ observed in sample is equal to or larger than critical value $X^{2*}$ or Find $p$ value corresponding to observed $X^2$ and check if it is equal to or smaller than $\alpha$	Two sided: Check if $z$ observed in sample is at least as extreme as critical value $z^$ or Find two sided $p$ value corresponding to observed $z$ and check if it is equal to or smaller than $\alpha$ Right sided: Check if $z$ observed in sample is equal to or larger than critical value $z^$ or Find right sided $p$ value corresponding to observed $z$ and check if it is equal to or smaller than $\alpha$ Left sided: Check if $z$ observed in sample is equal to or smaller than critical value $z^*$ or Find left sided $p$ value corresponding to observed $z$ and check if it is equal to or smaller than $\alpha$	If the number of blocks is large, the table with critical $X^2$ values can be used. If we denote $X^2 = Q$: Check if $X^2$ observed in sample is equal to or larger than critical value $X^{2*}$ or Find $p$ value corresponding to observed $X^2$ and check if it is equal to or smaller than $\alpha$
n.a.	n.a.	$C\%$ confidence interval for $\mu$	n.a.
-	-	$\bar{y} \pm z^* \times \dfrac{\sigma}{\sqrt{N}}$ where the critical value $z^$ is the value under the normal curve with the area $C / 100$ between $-z^$ and $z^$ (e.g. $z^$ = 1.96 for a 95% confidence interval). The confidence interval for $\mu$ can also be used as significance test.	-
Effect size	n.a.	Effect size	n.a.
Proportion variance explained $R^2$: Proportion variance of the dependent variable $y$ explained by the independent variables and the interaction effect together: $$ \begin{align} R^2 &= \dfrac{\mbox{sum of squares model}}{\mbox{sum of squares total}} \end{align} $$ $R^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population. Proportion variance explained $\eta^2$: Proportion variance of the dependent variable $y$ explained by an independent variable or interaction effect: $$ \begin{align} \eta^2_A &= \dfrac{\mbox{sum of squares A}}{\mbox{sum of squares total}}\\ \\ \eta^2_B &= \dfrac{\mbox{sum of squares B}}{\mbox{sum of squares total}}\\ \\ \eta^2_{int} &= \dfrac{\mbox{sum of squares int}}{\mbox{sum of squares total}} \end{align} $$ $\eta^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population. Proportion variance explained $\omega^2$: Corrects for the positive bias in $\eta^2$ and is equal to: $$ \begin{align} \omega^2_A &= \dfrac{\mbox{sum of squares A} - \mbox{degrees of freedom A} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \\ \omega^2_B &= \dfrac{\mbox{sum of squares B} - \mbox{degrees of freedom B} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \\ \omega^2_{int} &= \dfrac{\mbox{sum of squares int} - \mbox{degrees of freedom int} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \end{align} $$ $\omega^2$ is a better estimate of the explained variance in the population than $\eta^2$. Only for balanced designs (equal sample sizes). Proportion variance explained $\eta^2_{partial}$: $$ \begin{align} \eta^2_{partial\,A} &= \frac{\mbox{sum of squares A}}{\mbox{sum of squares A} + \mbox{sum of squares error}}\\ \\ \eta^2_{partial\,B} &= \frac{\mbox{sum of squares B}}{\mbox{sum of squares B} + \mbox{sum of squares error}}\\ \\ \eta^2_{partial\,int} &= \frac{\mbox{sum of squares int}}{\mbox{sum of squares int} + \mbox{sum of squares error}} \end{align} $$	-	Cohen's $d$: Standardized difference between the sample mean and $\mu_0$: $$d = \frac{\bar{y} - \mu_0}{\sigma}$$ Cohen's $d$ indicates how many standard deviations $\sigma$ the sample mean $\bar{y}$ is removed from $\mu_0.$	-
n.a.	n.a.	Visual representation	n.a.
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ANOVA table	n.a.	n.a.	n.a.
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Equivalent to	n.a.	n.a.	Equivalent to
OLS regression with two categorical independent variables and the interaction term, transformed into $(I - 1)$ + $(J - 1)$ + $(I - 1) \times (J - 1)$ code variables.	-	-	Friedman test, with a categorical dependent variable consisting of two independent groups.
Example context	Example context	Example context	Example context
Is the average mental health score different between people from a low, moderate, and high economic class? And is the average mental health score different between men and women? And is there an interaction effect between economic class and gender?	Is there an association between economic class and gender? Is the distribution of economic class different between men and women?	Is the average mental health score of office workers different from $\mu_0 = 50$? Assume that the standard deviation of the mental health scores in the population is $\sigma = 3.$	Subjects perform three different tasks, which they can either perform correctly or incorrectly. Is there a difference in task performance between the three different tasks?
SPSS	SPSS	n.a.	SPSS
Analyze > General Linear Model > Univariate... Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factor(s)	Analyze > Descriptive Statistics > Crosstabs... Put one of your two categorical variables in the box below Row(s), and the other categorical variable in the box below Column(s) Click the Statistics... button, and click on the square in front of Chi-square Continue and click OK	-	Analyze > Nonparametric Tests > Legacy Dialogs > K Related Samples... Put the $k$ variables containing the scores for the $k$ related groups in the white box below Test Variables Under Test Type, select Cochran's Q test
Jamovi	Jamovi	n.a.	Jamovi
ANOVA > ANOVA Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factors	Frequencies > Independent Samples - $\chi^2$ test of association Put one of your two categorical variables in the box below Rows, and the other categorical variable in the box below Columns	-	Jamovi does not have a specific option for the Cochran's Q test. However, you can do the Friedman test instead. The $p$ value resulting from this Friedman test is equivalent to the $p$ value that would have resulted from the Cochran's Q test. Go to: ANOVA > Repeated Measures ANOVA - Friedman Put the $k$ variables containing the scores for the $k$ related groups in the box below Measures
Practice questions	Practice questions	Practice questions	Practice questions

Two way ANOVA - overview