Two way ANOVA - overview

This page offers structured overviews of one or more selected methods. Add additional methods for comparisons (max. of 3) by clicking on the dropdown button in the right-hand column. To practice with a specific method click the button at the bottom row of the table

Two way ANOVA
Paired sample $t$ test
Kruskal-Wallis test
One sample $z$ test for the mean
Independent/grouping variablesIndependent variableIndependent/grouping variableIndependent variable
Two categorical, the first with $I$ independent groups and the second with $J$ independent groups ($I \geqslant 2$, $J \geqslant 2$)2 paired groupsOne categorical with $I$ independent groups ($I \geqslant 2$)None
Dependent variableDependent variableDependent variableDependent variable
One quantitative of interval or ratio levelOne quantitative of interval or ratio levelOne of ordinal levelOne quantitative of interval or ratio level
Null hypothesisNull hypothesisNull hypothesisNull hypothesis
ANOVA $F$ tests:
  • H0 for main and interaction effects together (model): no main effects and interaction effect
  • H0 for independent variable A: no main effect for A
  • H0 for independent variable B: no main effect for B
  • H0 for the interaction term: no interaction effect between A and B
Like in one way ANOVA, we can also perform $t$ tests for specific contrasts and multiple comparisons. This is more advanced stuff.
H0: $\mu = \mu_0$

Here $\mu$ is the population mean of the difference scores, and $\mu_0$ is the population mean of the difference scores according to the null hypothesis, which is usually 0. A difference score is the difference between the first score of a pair and the second score of a pair.
If the dependent variable is measured on a continuous scale and the shape of the distribution of the dependent variable is the same in all $I$ populations:
  • H0: the population medians for the $I$ groups are equal
Else:
Formulation 1:
  • H0: the population scores in any of the $I$ groups are not systematically higher or lower than the population scores in any of the other groups
Formulation 2:
  • H0: P(an observation from population $g$ exceeds an observation from population $h$) = P(an observation from population $h$ exceeds an observation from population $g$), for each pair of groups.
Several different formulations of the null hypothesis can be found in the literature, and we do not agree with all of them. Make sure you (also) learn the one that is given in your text book or by your teacher.
H0: $\mu = \mu_0$

Here $\mu$ is the population mean, and $\mu_0$ is the population mean according to the null hypothesis.
Alternative hypothesisAlternative hypothesisAlternative hypothesisAlternative hypothesis
ANOVA $F$ tests:
  • H1 for main and interaction effects together (model): there is a main effect for A, and/or for B, and/or an interaction effect
  • H1 for independent variable A: there is a main effect for A
  • H1 for independent variable B: there is a main effect for B
  • H1 for the interaction term: there is an interaction effect between A and B
H1 two sided: $\mu \neq \mu_0$
H1 right sided: $\mu > \mu_0$
H1 left sided: $\mu < \mu_0$
If the dependent variable is measured on a continuous scale and the shape of the distribution of the dependent variable is the same in all $I$ populations:
  • H1: not all of the population medians for the $I$ groups are equal
Else:
Formulation 1:
  • H1: the poplation scores in some groups are systematically higher or lower than the population scores in other groups
Formulation 2:
  • H1: for at least one pair of groups:
    P(an observation from population $g$ exceeds an observation from population $h$) $\neq$ P(an observation from population $h$ exceeds an observation from population $g$)
H1 two sided: $\mu \neq \mu_0$
H1 right sided: $\mu > \mu_0$
H1 left sided: $\mu < \mu_0$
AssumptionsAssumptionsAssumptionsAssumptions
  • Within each of the $I \times J$ populations, the scores on the dependent variable are normally distributed
  • The standard deviation of the scores on the dependent variable is the same in each of the $I \times J$ populations
  • For each of the $I \times J$ groups, the sample is an independent and simple random sample from the population defined by that group. That is, within and between groups, observations are independent of one another
  • Equal sample sizes for each group make the interpretation of the ANOVA output easier (unequal sample sizes result in overlap in the sum of squares; this is advanced stuff)
  • Difference scores are normally distributed in the population
  • Sample of difference scores is a simple random sample from the population of difference scores. That is, difference scores are independent of one another
  • Group 1 sample is a simple random sample (SRS) from population 1, group 2 sample is an independent SRS from population 2, $\ldots$, group $I$ sample is an independent SRS from population $I$. That is, within and between groups, observations are independent of one another
  • Scores are normally distributed in the population
  • Population standard deviation $\sigma$ is known
  • Sample is a simple random sample from the population. That is, observations are independent of one another
Test statisticTest statisticTest statisticTest statistic
For main and interaction effects together (model):
  • $F = \dfrac{\mbox{mean square model}}{\mbox{mean square error}}$
For independent variable A:
  • $F = \dfrac{\mbox{mean square A}}{\mbox{mean square error}}$
For independent variable B:
  • $F = \dfrac{\mbox{mean square B}}{\mbox{mean square error}}$
For the interaction term:
  • $F = \dfrac{\mbox{mean square interaction}}{\mbox{mean square error}}$
Note: mean square error is also known as mean square residual or mean square within.
$t = \dfrac{\bar{y} - \mu_0}{s / \sqrt{N}}$
Here $\bar{y}$ is the sample mean of the difference scores, $\mu_0$ is the population mean of the difference scores according to the null hypothesis, $s$ is the sample standard deviation of the difference scores, and $N$ is the sample size (number of difference scores).

The denominator $s / \sqrt{N}$ is the standard error of the sampling distribution of $\bar{y}$. The $t$ value indicates how many standard errors $\bar{y}$ is removed from $\mu_0$.

$H = \dfrac{12}{N (N + 1)} \sum \dfrac{R^2_i}{n_i} - 3(N + 1)$

Here $N$ is the total sample size, $R_i$ is the sum of ranks in group $i$, and $n_i$ is the sample size of group $i$. Remember that multiplication precedes addition, so first compute $\frac{12}{N (N + 1)} \times \sum \frac{R^2_i}{n_i}$ and then subtract $3(N + 1)$.

Note: if ties are present in the data, the formula for $H$ is more complicated.
$z = \dfrac{\bar{y} - \mu_0}{\sigma / \sqrt{N}}$
Here $\bar{y}$ is the sample mean, $\mu_0$ is the population mean according to the null hypothesis, $\sigma$ is the population standard deviation, and $N$ is the sample size.

The denominator $\sigma / \sqrt{N}$ is the standard deviation of the sampling distribution of $\bar{y}$. The $z$ value indicates how many of these standard deviations $\bar{y}$ is removed from $\mu_0$.
Pooled standard deviationn.a.n.a.n.a.
$ \begin{aligned} s_p &= \sqrt{\dfrac{\sum\nolimits_{subjects} (\mbox{subject's score} - \mbox{its group mean})^2}{N - (I \times J)}}\\ &= \sqrt{\dfrac{\mbox{sum of squares error}}{\mbox{degrees of freedom error}}}\\ &= \sqrt{\mbox{mean square error}} \end{aligned} $ ---
Sampling distribution of $F$ if H0 were trueSampling distribution of $t$ if H0 were trueSampling distribution of $H$ if H0 were trueSampling distribution of $z$ if H0 were true
For main and interaction effects together (model):
  • $F$ distribution with $(I - 1) + (J - 1) + (I - 1) \times (J - 1)$ (df model, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
For independent variable A:
  • $F$ distribution with $I - 1$ (df A, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
For independent variable B:
  • $F$ distribution with $J - 1$ (df B, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
For the interaction term:
  • $F$ distribution with $(I - 1) \times (J - 1)$ (df interaction, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
Here $N$ is the total sample size.
$t$ distribution with $N - 1$ degrees of freedom

For large samples, approximately the chi-squared distribution with $I - 1$ degrees of freedom.

For small samples, the exact distribution of $H$ should be used.

Standard normal distribution
Significant?Significant?Significant?Significant?
  • Check if $F$ observed in sample is equal to or larger than critical value $F^*$ or
  • Find $p$ value corresponding to observed $F$ and check if it is equal to or smaller than $\alpha$
Two sided: Right sided: Left sided: For large samples, the table with critical $X^2$ values can be used. If we denote $X^2 = H$:
  • Check if $X^2$ observed in sample is equal to or larger than critical value $X^{2*}$ or
  • Find $p$ value corresponding to observed $X^2$ and check if it is equal to or smaller than $\alpha$
Two sided: Right sided: Left sided:
n.a.$C\%$ confidence interval for $\mu$n.a.$C\%$ confidence interval for $\mu$
-$\bar{y} \pm t^* \times \dfrac{s}{\sqrt{N}}$
where the critical value $t^*$ is the value under the $t_{N-1}$ distribution with the area $C / 100$ between $-t^*$ and $t^*$ (e.g. $t^*$ = 2.086 for a 95% confidence interval when df = 20).

The confidence interval for $\mu$ can also be used as significance test.
-$\bar{y} \pm z^* \times \dfrac{\sigma}{\sqrt{N}}$
where the critical value $z^*$ is the value under the normal curve with the area $C / 100$ between $-z^*$ and $z^*$ (e.g. $z^*$ = 1.96 for a 95% confidence interval).

The confidence interval for $\mu$ can also be used as significance test.
Effect sizeEffect sizen.a.Effect size
  • Proportion variance explained $R^2$:
    Proportion variance of the dependent variable $y$ explained by the independent variables and the interaction effect together:
    $$ \begin{align} R^2 &= \dfrac{\mbox{sum of squares model}}{\mbox{sum of squares total}} \end{align} $$ $R^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population.

  • Proportion variance explained $\eta^2$:
    Proportion variance of the dependent variable $y$ explained by an independent variable or interaction effect:
    $$ \begin{align} \eta^2_A &= \dfrac{\mbox{sum of squares A}}{\mbox{sum of squares total}}\\ \\ \eta^2_B &= \dfrac{\mbox{sum of squares B}}{\mbox{sum of squares total}}\\ \\ \eta^2_{int} &= \dfrac{\mbox{sum of squares int}}{\mbox{sum of squares total}} \end{align} $$ $\eta^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population.

  • Proportion variance explained $\omega^2$:
    Corrects for the positive bias in $\eta^2$ and is equal to:
    $$ \begin{align} \omega^2_A &= \dfrac{\mbox{sum of squares A} - \mbox{degrees of freedom A} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \\ \omega^2_B &= \dfrac{\mbox{sum of squares B} - \mbox{degrees of freedom B} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \\ \omega^2_{int} &= \dfrac{\mbox{sum of squares int} - \mbox{degrees of freedom int} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \end{align} $$ $\omega^2$ is a better estimate of the explained variance in the population than $\eta^2$. Only for balanced designs (equal sample sizes).

  • Proportion variance explained $\eta^2_{partial}$: $$ \begin{align} \eta^2_{partial\,A} &= \frac{\mbox{sum of squares A}}{\mbox{sum of squares A} + \mbox{sum of squares error}}\\ \\ \eta^2_{partial\,B} &= \frac{\mbox{sum of squares B}}{\mbox{sum of squares B} + \mbox{sum of squares error}}\\ \\ \eta^2_{partial\,int} &= \frac{\mbox{sum of squares int}}{\mbox{sum of squares int} + \mbox{sum of squares error}} \end{align} $$
Cohen's $d$:
Standardized difference between the sample mean of the difference scores and $\mu_0$: $$d = \frac{\bar{y} - \mu_0}{s}$$ Cohen's $d$ indicates how many standard deviations $s$ the sample mean of the difference scores $\bar{y}$ is removed from $\mu_0.$
-Cohen's $d$:
Standardized difference between the sample mean and $\mu_0$: $$d = \frac{\bar{y} - \mu_0}{\sigma}$$ Cohen's $d$ indicates how many standard deviations $\sigma$ the sample mean $\bar{y}$ is removed from $\mu_0.$
n.a.Visual representationn.a.Visual representation
-
Paired sample t test
-
One sample z test
ANOVA tablen.a.n.a.n.a.
two way ANOVA table
---
Equivalent toEquivalent ton.a.n.a.
OLS regression with two categorical independent variables and the interaction term, transformed into $(I - 1)$ + $(J - 1)$ + $(I - 1) \times (J - 1)$ code variables.
  • One sample $t$ test on the difference scores.
  • Repeated measures ANOVA with one dichotomous within subjects factor.
--
Example contextExample contextExample contextExample context
Is the average mental health score different between people from a low, moderate, and high economic class? And is the average mental health score different between men and women? And is there an interaction effect between economic class and gender?Is the average difference between the mental health scores before and after an intervention different from $\mu_0 = 0$?Do people from different religions tend to score differently on social economic status? Is the average mental health score of office workers different from $\mu_0 = 50$? Assume that the standard deviation of the mental health scores in the population is $\sigma = 3.$
SPSSSPSSSPSSn.a.
Analyze > General Linear Model > Univariate...
  • Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factor(s)
Analyze > Compare Means > Paired-Samples T Test...
  • Put the two paired variables in the boxes below Variable 1 and Variable 2
Analyze > Nonparametric Tests > Legacy Dialogs > K Independent Samples...
  • Put your dependent variable in the box below Test Variable List and your independent (grouping) variable in the box below Grouping Variable
  • Click on the Define Range... button. If you can't click on it, first click on the grouping variable so its background turns yellow
  • Fill in the smallest value you have used to indicate your groups in the box next to Minimum, and the largest value you have used to indicate your groups in the box next to Maximum
  • Continue and click OK
-
JamoviJamoviJamovin.a.
ANOVA > ANOVA
  • Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factors
T-Tests > Paired Samples T-Test
  • Put the two paired variables in the box below Paired Variables, one on the left side of the vertical line and one on the right side of the vertical line
  • Under Hypothesis, select your alternative hypothesis
ANOVA > One Way ANOVA - Kruskal-Wallis
  • Put your dependent variable in the box below Dependent Variables and your independent (grouping) variable in the box below Grouping Variable
-
Practice questionsPractice questionsPractice questionsPractice questions