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Two categorical, the first with $I$ independent groups and the second with $J$ independent groups ($I \geqslant 2$, $J \geqslant 2$)
Dependent variable
Dependent variable
One quantitative of interval or ratio level
One quantitative of interval or ratio level
Null hypothesis
Null hypothesis
H_{0}: $m = 0$
Here $m$ is the population median of the difference scores. A difference score is the difference between the first score of a pair and the second score of a pair.
Several different formulations of the null hypothesis can be found in the literature, and we do not agree with all of them. Make sure you (also) learn the one that is given in your text book or by your teacher.
ANOVA $F$ tests:
H_{0} for main and interaction effects together (model): no main effects and interaction effect
H_{0} for independent variable A: no main effect for A
H_{0} for independent variable B: no main effect for B
H_{0} for the interaction term: no interaction effect between A and B
Like in one way ANOVA, we can also perform $t$ tests for specific contrasts and multiple comparisons. This is more advanced stuff.
Alternative hypothesis
Alternative hypothesis
H_{1} two sided: $m \neq 0$
H_{1} right sided: $m > 0$
H_{1} left sided: $m < 0$
ANOVA $F$ tests:
H_{1} for main and interaction effects together (model): there is a main effect for A, and/or for B, and/or an interaction effect
H_{1} for independent variable A: there is a main effect for A
H_{1} for independent variable B: there is a main effect for B
H_{1} for the interaction term: there is an interaction effect between A and B
Assumptions
Assumptions
The population distribution of the difference scores is symmetric
Sample of difference scores is a simple random sample from the population of difference scores. That is, difference scores are independent of one another
Note: sometimes it considered sufficient for the data to be measured on an ordinal scale, rather than an interval or ratio scale. However, since the test statistic is based on ranked difference scores, we need to know whether a change in scores from, say, 6 to 7 is larger than/smaller than/equal to a change from 5 to 6. This is impossible to know for ordinal scales, since for these scales the size of the difference between values is meaningless.
Within each of the $I \times J$ populations, the scores on the dependent variable are normally distributed
The standard deviation of the scores on the dependent variable is the same in each of the $I \times J$ populations
For each of the $I \times J$ groups, the sample is an independent and simple random sample from the population defined by that group. That is, within and between groups, observations are independent of one another
Equal sample sizes for each group make the interpretation of the ANOVA output easier (unequal sample sizes result in overlap in the sum of squares; this is advanced stuff)
Test statistic
Test statistic
Two different types of test statistics can be used, but both will result in the same test outcome. We will denote the first option the $W_1$ statistic (also known as the $T$ statistic), and the second option the $W_2$ statistic.
In order to compute each of the test statistics, follow the steps below:
For each subject, compute the sign of the difference score $\mbox{sign}_d = \mbox{sgn}(\mbox{score}_2  \mbox{score}_1)$. The sign is 1 if the difference is larger than zero, 1 if the diffence is smaller than zero, and 0 if the difference is equal to zero.
For each subject, compute the absolute value of the difference score $\mbox{score}_2  \mbox{score}_1$.
Exclude subjects with a difference score of zero. This leaves us with a remaining number of difference scores equal to $N_r$.
Assign ranks $R_d$ to the $N_r$ remaining absolute difference scores. The smallest absolute difference score corresponds to a rank score of 1, and the largest absolute difference score corresponds to a rank score of $N_r$. If there are ties, assign them the average of the ranks they occupy.
Then compute the test statistic:
$W_1 = \sum\, R_d^{+}$
or
$W_1 = \sum\, R_d^{}$
That is, sum all ranks corresponding to a positive difference or sum all ranks corresponding to a negative difference. Theoratically, both definitions will result in the same test outcome. However:
tables with critical values for $W_1$ are usually based on the smaller of $\sum\, R_d^{+}$ and $\sum\, R_d^{}$. So if you are using such a table, pick the smaller one.
If you are using the normal approximation to find the $p$ value, it makes things most straightforward if you use $W_1 = \sum\, R_d^{+}$ (if you use $W_1 = \sum\, R_d^{}$, the right and left sided alternative hypotheses 'flip').
$W_2 = \sum\, \mbox{sign}_d \times R_d$
That is, for each remaining difference score, multiply the rank of the absolute difference score by the sign of the difference score, and then sum all of the products.
For main and interaction effects together (model):
Note: mean square error is also known as mean square residual or mean square within.
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Pooled standard deviation

$
\begin{aligned}
s_p &= \sqrt{\dfrac{\sum\nolimits_{subjects} (\mbox{subject's score}  \mbox{its group mean})^2}{N  (I \times J)}}\\
&= \sqrt{\dfrac{\mbox{sum of squares error}}{\mbox{degrees of freedom error}}}\\
&= \sqrt{\mbox{mean square error}}
\end{aligned}
$
Sampling distribution of $W_1$ and of $W_2$ if H_{0} were true
Sampling distribution of $F$ if H_{0} were true
Sampling distribution of $W_1$:
If $N_r$ is large, $W_1$ is approximately normally distributed with mean $\mu_{W_1}$ and standard deviation $\sigma_{W_1}$ if the null hypothesis were true. Here
$$\mu_{W_1} = \frac{N_r(N_r + 1)}{4}$$
$$\sigma_{W_1} = \sqrt{\frac{N_r(N_r + 1)(2N_r + 1)}{24}}$$
Hence, if $N_r$ is large, the standardized test statistic
$$z = \frac{W_1  \mu_{W_1}}{\sigma_{W_1}}$$
follows approximately the standard normal distribution if the null hypothesis were true.
Sampling distribution of $W_2$:
If $N_r$ is large, $W_2$ is approximately normally distributed with mean $0$ and standard deviation $\sigma_{W_2}$ if the null hypothesis were true. Here
$$\sigma_{W_2} = \sqrt{\frac{N_r(N_r + 1)(2N_r + 1)}{6}}$$
Hence, if $N_r$ is large, the standardized test statistic
$$z = \frac{W_2}{\sigma_{W_2}}$$
follows approximately the standard normal distribution if the null hypothesis were true.
If $N_r$ is small, the exact distribution of $W_1$ or $W_2$ should be used.
Note: if ties are present in the data, the formula for the standard deviations $\sigma_{W_1}$ and $\sigma_{W_2}$ is more complicated.
For main and interaction effects together (model):
$F$ distribution with $(I  1) + (J  1) + (I  1) \times (J  1)$ (df model, numerator) and $N  (I \times J)$ (df error, denominator) degrees of freedom
For independent variable A:
$F$ distribution with $I  1$ (df A, numerator) and $N  (I \times J)$ (df error, denominator) degrees of freedom
For independent variable B:
$F$ distribution with $J  1$ (df B, numerator) and $N  (I \times J)$ (df error, denominator) degrees of freedom
For the interaction term:
$F$ distribution with $(I  1) \times (J  1)$ (df interaction, numerator) and $N  (I \times J)$ (df error, denominator) degrees of freedom
Here $N$ is the total sample size.
Significant?
Significant?
For large samples, the table for standard normal probabilities can be used:
Two sided:
Check if $z$ observed in sample is at least as extreme as critical value $z^*$ or
Find two sided $p$ value corresponding to observed $z$ and check if it is equal to or smaller than $\alpha$
Right sided:
Check if $z$ observed in sample is equal to or larger than critical value $z^*$ or
Find right sided $p$ value corresponding to observed $z$ and check if it is equal to or smaller than $\alpha$
Left sided:
Check if $z$ observed in sample is equal to or smaller than critical value $z^*$ or
Find left sided $p$ value corresponding to observed $z$ and check if it is equal to or smaller than $\alpha$
Check if $F$ observed in sample is equal to or larger than critical value $F^*$ or
Find $p$ value corresponding to observed $F$ and check if it is equal to or smaller than $\alpha$
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Effect size

Proportion variance explained $R^2$:
Proportion variance of the dependent variable $y$ explained by the independent variables and the interaction effect together:
$$
\begin{align}
R^2 &= \dfrac{\mbox{sum of squares model}}{\mbox{sum of squares total}}
\end{align}
$$
$R^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population.
Proportion variance explained $\eta^2$:
Proportion variance of the dependent variable $y$ explained by an independent variable or interaction effect:
$$
\begin{align}
\eta^2_A &= \dfrac{\mbox{sum of squares A}}{\mbox{sum of squares total}}\\
\\
\eta^2_B &= \dfrac{\mbox{sum of squares B}}{\mbox{sum of squares total}}\\
\\
\eta^2_{int} &= \dfrac{\mbox{sum of squares int}}{\mbox{sum of squares total}}
\end{align}
$$
$\eta^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population.
Proportion variance explained $\omega^2$:
Corrects for the positive bias in $\eta^2$ and is equal to:
$$
\begin{align}
\omega^2_A &= \dfrac{\mbox{sum of squares A}  \mbox{degrees of freedom A} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\
\\
\omega^2_B &= \dfrac{\mbox{sum of squares B}  \mbox{degrees of freedom B} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\
\\
\omega^2_{int} &= \dfrac{\mbox{sum of squares int}  \mbox{degrees of freedom int} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\
\end{align}
$$
$\omega^2$ is a better estimate of the explained variance in the population than
$\eta^2$. Only for balanced designs (equal sample sizes).
Proportion variance explained $\eta^2_{partial}$:
$$
\begin{align}
\eta^2_{partial\,A} &= \frac{\mbox{sum of squares A}}{\mbox{sum of squares A} + \mbox{sum of squares error}}\\
\\
\eta^2_{partial\,B} &= \frac{\mbox{sum of squares B}}{\mbox{sum of squares B} + \mbox{sum of squares error}}\\
\\
\eta^2_{partial\,int} &= \frac{\mbox{sum of squares int}}{\mbox{sum of squares int} + \mbox{sum of squares error}}
\end{align}
$$
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ANOVA table

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Equivalent to

OLS regression with two categorical independent variables and the interaction term, transformed into $(I  1)$ + $(J  1)$ + $(I  1) \times (J  1)$ code variables.
Example context
Example context
Is the median of the differences between the mental health scores before and after an intervention different from 0?
Is the average mental health score different between people from a low, moderate, and high economic class? And is the average mental health score different between men and women? And is there an interaction effect between economic class and gender?
Put the two paired variables in the boxes below Variable 1 and Variable 2
Under Test Type, select the Wilcoxon test
Analyze > General Linear Model > Univariate...
Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factor(s)
Jamovi
Jamovi
TTests > Paired Samples TTest
Put the two paired variables in the box below Paired Variables, one on the left side of the vertical line and one on the right side of the vertical line
Under Tests, select Wilcoxon rank
Under Hypothesis, select your alternative hypothesis
ANOVA > ANOVA
Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factors