# Two sample t test - equal variances not assumed - overview

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Two sample $t$ test - equal variances not assumed
Spearman's rho
Mann-Whitney-Wilcoxon test
Independent/grouping variableVariable 1Independent/grouping variable
One categorical with 2 independent groupsOne of ordinal levelOne categorical with 2 independent groups
Dependent variableVariable 2Dependent variable
One quantitative of interval or ratio levelOne of ordinal levelOne of ordinal level
Null hypothesisNull hypothesisNull hypothesis
H0: $\mu_1 = \mu_2$

Here $\mu_1$ is the population mean for group 1, and $\mu_2$ is the population mean for group 2.
H0: $\rho_s = 0$

Here $\rho_s$ is the Spearman correlation in the population. The Spearman correlation is a measure for the strength and direction of the monotonic relationship between two variables of at least ordinal measurement level.

In words, the null hypothesis would be:

H0: there is no monotonic relationship between the two variables in the population.
If the dependent variable is measured on a continuous scale and the shape of the distribution of the dependent variable is the same in both populations:
• H0: the population median for group 1 is equal to the population median for group 2
Else:
Formulation 1:
• H0: the population scores in group 1 are not systematically higher or lower than the population scores in group 2
Formulation 2:
• H0: P(an observation from population 1 exceeds an observation from population 2) = P(an observation from population 2 exceeds observation from population 1)
Several different formulations of the null hypothesis can be found in the literature, and we do not agree with all of them. Make sure you (also) learn the one that is given in your text book or by your teacher.
Alternative hypothesisAlternative hypothesisAlternative hypothesis
H1 two sided: $\mu_1 \neq \mu_2$
H1 right sided: $\mu_1 > \mu_2$
H1 left sided: $\mu_1 < \mu_2$
H1 two sided: $\rho_s \neq 0$
H1 right sided: $\rho_s > 0$
H1 left sided: $\rho_s < 0$
If the dependent variable is measured on a continuous scale and the shape of the distribution of the dependent variable is the same in both populations:
• H1 two sided: the population median for group 1 is not equal to the population median for group 2
• H1 right sided: the population median for group 1 is larger than the population median for group 2
• H1 left sided: the population median for group 1 is smaller than the population median for group 2
Else:
Formulation 1:
• H1 two sided: the population scores in group 1 are systematically higher or lower than the population scores in group 2
• H1 right sided: the population scores in group 1 are systematically higher than the population scores in group 2
• H1 left sided: the population scores in group 1 are systematically lower than the population scores in group 2
Formulation 2:
• H1 two sided: P(an observation from population 1 exceeds an observation from population 2) $\neq$ P(an observation from population 2 exceeds an observation from population 1)
• H1 right sided: P(an observation from population 1 exceeds an observation from population 2) > P(an observation from population 2 exceeds an observation from population 1)
• H1 left sided: P(an observation from population 1 exceeds an observation from population 2) < P(an observation from population 2 exceeds an observation from population 1)
AssumptionsAssumptionsAssumptions
• Within each population, the scores on the dependent variable are normally distributed
• Group 1 sample is a simple random sample (SRS) from population 1, group 2 sample is an independent SRS from population 2. That is, within and between groups, observations are independent of one another
• Sample of pairs is a simple random sample from the population of pairs. That is, pairs are independent of one another
Note: this assumption is only important for the significance test, not for the correlation coefficient itself. The correlation coefficient itself just measures the strength of the monotonic relationship between two variables.
• Group 1 sample is a simple random sample (SRS) from population 1, group 2 sample is an independent SRS from population 2. That is, within and between groups, observations are independent of one another
Test statisticTest statisticTest statistic
$t = \dfrac{(\bar{y}_1 - \bar{y}_2) - 0}{\sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}} = \dfrac{\bar{y}_1 - \bar{y}_2}{\sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}}$
Here $\bar{y}_1$ is the sample mean in group 1, $\bar{y}_2$ is the sample mean in group 2, $s^2_1$ is the sample variance in group 1, $s^2_2$ is the sample variance in group 2, $n_1$ is the sample size of group 1, and $n_2$ is the sample size of group 2. The 0 represents the difference in population means according to the null hypothesis.

The denominator $\sqrt{\frac{s^2_1}{n_1} + \frac{s^2_2}{n_2}}$ is the standard error of the sampling distribution of $\bar{y}_1 - \bar{y}_2$. The $t$ value indicates how many standard errors $\bar{y}_1 - \bar{y}_2$ is removed from 0.

Note: we could just as well compute $\bar{y}_2 - \bar{y}_1$ in the numerator, but then the left sided alternative becomes $\mu_2 < \mu_1$, and the right sided alternative becomes $\mu_2 > \mu_1$.
$t = \dfrac{r_s \times \sqrt{N - 2}}{\sqrt{1 - r_s^2}}$
Here $r_s$ is the sample Spearman correlation and $N$ is the sample size. The sample Spearman correlation $r_s$ is equal to the Pearson correlation applied to the rank scores.
Two different types of test statistics can be used; both will result in the same test outcome. The first is the Wilcoxon rank sum statistic $W$:
The second type of test statistic is the Mann-Whitney $U$ statistic:
• $U = W - \dfrac{n_1(n_1 + 1)}{2}$
where $n_1$ is the sample size of group 1.

Note: we could just as well base W and U on group 2. This would only 'flip' the right and left sided alternative hypotheses. Also, tables with critical values for $U$ are often based on the smaller of $U$ for group 1 and for group 2.
Sampling distribution of $t$ if H0 were trueSampling distribution of $t$ if H0 were trueSampling distribution of $W$ and of $U$ if H0 were true
Approximately the $t$ distribution with $k$ degrees of freedom, with $k$ equal to
$k = \dfrac{\Bigg(\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}\Bigg)^2}{\dfrac{1}{n_1 - 1} \Bigg(\dfrac{s^2_1}{n_1}\Bigg)^2 + \dfrac{1}{n_2 - 1} \Bigg(\dfrac{s^2_2}{n_2}\Bigg)^2}$
or
$k$ = the smaller of $n_1$ - 1 and $n_2$ - 1

First definition of $k$ is used by computer programs, second definition is often used for hand calculations.
Approximately the $t$ distribution with $N - 2$ degrees of freedom

Sampling distribution of $W$:
For large samples, $W$ is approximately normally distributed with mean $\mu_W$ and standard deviation $\sigma_W$ if the null hypothesis were true. Here \begin{aligned} \mu_W &= \dfrac{n_1(n_1 + n_2 + 1)}{2}\\ \sigma_W &= \sqrt{\dfrac{n_1 n_2(n_1 + n_2 + 1)}{12}} \end{aligned} Hence, for large samples, the standardized test statistic $$z_W = \dfrac{W - \mu_W}{\sigma_W}\\$$ follows approximately the standard normal distribution if the null hypothesis were true. Note that if your $W$ value is based on group 2, $\mu_W$ becomes $\frac{n_2(n_1 + n_2 + 1)}{2}$.

Sampling distribution of $U$:
For large samples, $U$ is approximately normally distributed with mean $\mu_U$ and standard deviation $\sigma_U$ if the null hypothesis were true. Here \begin{aligned} \mu_U &= \dfrac{n_1 n_2}{2}\\ \sigma_U &= \sqrt{\dfrac{n_1 n_2(n_1 + n_2 + 1)}{12}} \end{aligned} Hence, for large samples, the standardized test statistic $$z_U = \dfrac{U - \mu_U}{\sigma_U}\\$$ follows approximately the standard normal distribution if the null hypothesis were true.

For small samples, the exact distribution of $W$ or $U$ should be used.

Note: if ties are present in the data, the formula for the standard deviations $\sigma_W$ and $\sigma_U$ is more complicated.
Significant?Significant?Significant?
Two sided:
Right sided:
Left sided:
Two sided:
Right sided:
Left sided:
For large samples, the table for standard normal probabilities can be used:
Two sided:
Right sided:
Left sided:
Approximate $C\%$ confidence interval for $\mu_1 - \mu_2$n.a.n.a.
$(\bar{y}_1 - \bar{y}_2) \pm t^* \times \sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}$
where the critical value $t^*$ is the value under the $t_{k}$ distribution with the area $C / 100$ between $-t^*$ and $t^*$ (e.g. $t^*$ = 2.086 for a 95% confidence interval when df = 20).

The confidence interval for $\mu_1 - \mu_2$ can also be used as significance test.
--
Visual representationn.a.n.a.
--
n.a.n.a.Equivalent to
--If there are no ties in the data, the two sided Mann-Whitney-Wilcoxon test is equivalent to the Kruskal-Wallis test with an independent variable with 2 levels ($I$ = 2).
Example contextExample contextExample context
Is the average mental health score different between men and women?Is there a monotonic relationship between physical health and mental health?Do men tend to score higher on social economic status than women?
SPSSSPSSSPSS
Analyze > Compare Means > Independent-Samples T Test...
• Put your dependent (quantitative) variable in the box below Test Variable(s) and your independent (grouping) variable in the box below Grouping Variable
• Click on the Define Groups... button. If you can't click on it, first click on the grouping variable so its background turns yellow
• Fill in the value you have used to indicate your first group in the box next to Group 1, and the value you have used to indicate your second group in the box next to Group 2
• Continue and click OK
Analyze > Correlate > Bivariate...
• Put your two variables in the box below Variables
• Under Correlation Coefficients, select Spearman
Analyze > Nonparametric Tests > Legacy Dialogs > 2 Independent Samples...
• Put your dependent variable in the box below Test Variable List and your independent (grouping) variable in the box below Grouping Variable
• Click on the Define Groups... button. If you can't click on it, first click on the grouping variable so its background turns yellow
• Fill in the value you have used to indicate your first group in the box next to Group 1, and the value you have used to indicate your second group in the box next to Group 2
• Continue and click OK
JamoviJamoviJamovi
T-Tests > Independent Samples T-Test
• Put your dependent (quantitative) variable in the box below Dependent Variables and your independent (grouping) variable in the box below Grouping Variable
• Under Tests, select Welch's
• Under Hypothesis, select your alternative hypothesis
Regression > Correlation Matrix
• Put your two variables in the white box at the right
• Under Correlation Coefficients, select Spearman
• Under Hypothesis, select your alternative hypothesis
T-Tests > Independent Samples T-Test
• Put your dependent variable in the box below Dependent Variables and your independent (grouping) variable in the box below Grouping Variable
• Under Tests, select Mann-Whitney U
• Under Hypothesis, select your alternative hypothesis
Practice questionsPractice questionsPractice questions