# Two sample t test - equal variances not assumed - overview

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Two sample $t$ test - equal variances not assumed
Spearman's rho
McNemar's test
One way ANOVA
Independent/grouping variableVariable 1Independent variableIndependent/grouping variable
One categorical with 2 independent groupsOne of ordinal level2 paired groupsOne categorical with $I$ independent groups ($I \geqslant 2$)
Dependent variableVariable 2Dependent variableDependent variable
One quantitative of interval or ratio levelOne of ordinal levelOne categorical with 2 independent groupsOne quantitative of interval or ratio level
Null hypothesisNull hypothesisNull hypothesisNull hypothesis
H0: $\mu_1 = \mu_2$

Here $\mu_1$ is the population mean for group 1, and $\mu_2$ is the population mean for group 2.
H0: $\rho_s = 0$

Here $\rho_s$ is the Spearman correlation in the population. The Spearman correlation is a measure for the strength and direction of the monotonic relationship between two variables of at least ordinal measurement level.

In words, the null hypothesis would be:

H0: there is no monotonic relationship between the two variables in the population.

Let's say that the scores on the dependent variable are scored 0 and 1. Then for each pair of scores, the data allow four options:

1. First score of pair is 0, second score of pair is 0
2. First score of pair is 0, second score of pair is 1 (switched)
3. First score of pair is 1, second score of pair is 0 (switched)
4. First score of pair is 1, second score of pair is 1
The null hypothesis H0 is that for each pair of scores, P(first score of pair is 0 while second score of pair is 1) = P(first score of pair is 1 while second score of pair is 0). That is, the probability that a pair of scores switches from 0 to 1 is the same as the probability that a pair of scores switches from 1 to 0.

Other formulations of the null hypothesis are:

• H0: $\pi_1 = \pi_2$, where $\pi_1$ is the population proportion of ones for the first paired group and $\pi_2$ is the population proportion of ones for the second paired group
• H0: for each pair of scores, P(first score of pair is 1) = P(second score of pair is 1)

ANOVA $F$ test:
• H0: $\mu_1 = \mu_2 = \ldots = \mu_I$
$\mu_1$ is the population mean for group 1; $\mu_2$ is the population mean for group 2; $\mu_I$ is the population mean for group $I$
$t$ Test for contrast:
• H0: $\Psi = 0$
$\Psi$ is the population contrast, defined as $\Psi = \sum a_i\mu_i$. Here $\mu_i$ is the population mean for group $i$ and $a_i$ is the coefficient for $\mu_i$. The coefficients $a_i$ sum to 0.
$t$ Test multiple comparisons:
• H0: $\mu_g = \mu_h$
$\mu_g$ is the population mean for group $g$; $\mu_h$ is the population mean for group $h$
Alternative hypothesisAlternative hypothesisAlternative hypothesisAlternative hypothesis
H1 two sided: $\mu_1 \neq \mu_2$
H1 right sided: $\mu_1 > \mu_2$
H1 left sided: $\mu_1 < \mu_2$
H1 two sided: $\rho_s \neq 0$
H1 right sided: $\rho_s > 0$
H1 left sided: $\rho_s < 0$

The alternative hypothesis H1 is that for each pair of scores, P(first score of pair is 0 while second score of pair is 1) $\neq$ P(first score of pair is 1 while second score of pair is 0). That is, the probability that a pair of scores switches from 0 to 1 is not the same as the probability that a pair of scores switches from 1 to 0.

Other formulations of the alternative hypothesis are:

• H1: $\pi_1 \neq \pi_2$
• H1: for each pair of scores, P(first score of pair is 1) $\neq$ P(second score of pair is 1)

ANOVA $F$ test:
• H1: not all population means are equal
$t$ Test for contrast:
• H1 two sided: $\Psi \neq 0$
• H1 right sided: $\Psi > 0$
• H1 left sided: $\Psi < 0$
$t$ Test multiple comparisons:
• H1 - usually two sided: $\mu_g \neq \mu_h$
AssumptionsAssumptionsAssumptionsAssumptions
• Within each population, the scores on the dependent variable are normally distributed
• Group 1 sample is a simple random sample (SRS) from population 1, group 2 sample is an independent SRS from population 2. That is, within and between groups, observations are independent of one another
• Sample of pairs is a simple random sample from the population of pairs. That is, pairs are independent of one another
Note: this assumption is only important for the significance test, not for the correlation coefficient itself. The correlation coefficient itself just measures the strength of the monotonic relationship between two variables.
• Sample of pairs is a simple random sample from the population of pairs. That is, pairs are independent of one another
• Within each population, the scores on the dependent variable are normally distributed
• The standard deviation of the scores on the dependent variable is the same in each of the populations: $\sigma_1 = \sigma_2 = \ldots = \sigma_I$
• Group 1 sample is a simple random sample (SRS) from population 1, group 2 sample is an independent SRS from population 2, $\ldots$, group $I$ sample is an independent SRS from population $I$. That is, within and between groups, observations are independent of one another
Test statisticTest statisticTest statisticTest statistic
$t = \dfrac{(\bar{y}_1 - \bar{y}_2) - 0}{\sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}} = \dfrac{\bar{y}_1 - \bar{y}_2}{\sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}}$
Here $\bar{y}_1$ is the sample mean in group 1, $\bar{y}_2$ is the sample mean in group 2, $s^2_1$ is the sample variance in group 1, $s^2_2$ is the sample variance in group 2, $n_1$ is the sample size of group 1, and $n_2$ is the sample size of group 2. The 0 represents the difference in population means according to the null hypothesis.

The denominator $\sqrt{\frac{s^2_1}{n_1} + \frac{s^2_2}{n_2}}$ is the standard error of the sampling distribution of $\bar{y}_1 - \bar{y}_2$. The $t$ value indicates how many standard errors $\bar{y}_1 - \bar{y}_2$ is removed from 0.

Note: we could just as well compute $\bar{y}_2 - \bar{y}_1$ in the numerator, but then the left sided alternative becomes $\mu_2 < \mu_1$, and the right sided alternative becomes $\mu_2 > \mu_1$.
$t = \dfrac{r_s \times \sqrt{N - 2}}{\sqrt{1 - r_s^2}}$
Here $r_s$ is the sample Spearman correlation and $N$ is the sample size. The sample Spearman correlation $r_s$ is equal to the Pearson correlation applied to the rank scores.
$X^2 = \dfrac{(b - c)^2}{b + c}$
Here $b$ is the number of pairs in the sample for which the first score is 0 while the second score is 1, and $c$ is the number of pairs in the sample for which the first score is 1 while the second score is 0.
ANOVA $F$ test:
• \begin{aligned}[t] F &= \dfrac{\sum\nolimits_{subjects} (\mbox{subject's group mean} - \mbox{overall mean})^2 / (I - 1)}{\sum\nolimits_{subjects} (\mbox{subject's score} - \mbox{its group mean})^2 / (N - I)}\\ &= \dfrac{\mbox{sum of squares between} / \mbox{degrees of freedom between}}{\mbox{sum of squares error} / \mbox{degrees of freedom error}}\\ &= \dfrac{\mbox{mean square between}}{\mbox{mean square error}} \end{aligned}
where $N$ is the total sample size, and $I$ is the number of groups.
Note: mean square between is also known as mean square model, and mean square error is also known as mean square residual or mean square within.
$t$ Test for contrast:
• $t = \dfrac{c}{s_p\sqrt{\sum \dfrac{a^2_i}{n_i}}}$
Here $c$ is the sample estimate of the population contrast $\Psi$: $c = \sum a_i\bar{y}_i$, with $\bar{y}_i$ the sample mean in group $i$. $s_p$ is the pooled standard deviation based on all the $I$ groups in the ANOVA, $a_i$ is the contrast coefficient for group $i$, and $n_i$ is the sample size of group $i$.
Note that if the contrast compares only two group means with each other, this $t$ statistic is very similar to the two sample $t$ statistic (assuming equal population standard deviations). In that case the only difference is that we now base the pooled standard deviation on all the $I$ groups, which affects the $t$ value if $I \geqslant 3$. It also affects the corresponding degrees of freedom.
$t$ Test multiple comparisons:
• $t = \dfrac{\bar{y}_g - \bar{y}_h}{s_p\sqrt{\dfrac{1}{n_g} + \dfrac{1}{n_h}}}$
$\bar{y}_g$ is the sample mean in group $g$, $\bar{y}_h$ is the sample mean in group $h$, $s_p$ is the pooled standard deviation based on all the $I$ groups in the ANOVA, $n_g$ is the sample size of group $g$, and $n_h$ is the sample size of group $h$.
Note that this $t$ statistic is very similar to the two sample $t$ statistic (assuming equal population standard deviations). The only difference is that we now base the pooled standard deviation on all the $I$ groups, which affects the $t$ value if $I \geqslant 3$. It also affects the corresponding degrees of freedom.
n.a.n.a.n.a.Pooled standard deviation
---\begin{aligned} s_p &= \sqrt{\dfrac{(n_1 - 1) \times s^2_1 + (n_2 - 1) \times s^2_2 + \ldots + (n_I - 1) \times s^2_I}{N - I}}\\ &= \sqrt{\dfrac{\sum\nolimits_{subjects} (\mbox{subject's score} - \mbox{its group mean})^2}{N - I}}\\ &= \sqrt{\dfrac{\mbox{sum of squares error}}{\mbox{degrees of freedom error}}}\\ &= \sqrt{\mbox{mean square error}} \end{aligned}

Here $s^2_i$ is the variance in group $i.$
Sampling distribution of $t$ if H0 were trueSampling distribution of $t$ if H0 were trueSampling distribution of $X^2$ if H0 were trueSampling distribution of $F$ and of $t$ if H0 were true
Approximately the $t$ distribution with $k$ degrees of freedom, with $k$ equal to
$k = \dfrac{\Bigg(\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}\Bigg)^2}{\dfrac{1}{n_1 - 1} \Bigg(\dfrac{s^2_1}{n_1}\Bigg)^2 + \dfrac{1}{n_2 - 1} \Bigg(\dfrac{s^2_2}{n_2}\Bigg)^2}$
or
$k$ = the smaller of $n_1$ - 1 and $n_2$ - 1

First definition of $k$ is used by computer programs, second definition is often used for hand calculations.
Approximately the $t$ distribution with $N - 2$ degrees of freedom

If $b + c$ is large enough (say, > 20), approximately the chi-squared distribution with 1 degree of freedom.

If $b + c$ is small, the Binomial($n$, $P$) distribution should be used, with $n = b + c$ and $P = 0.5$. In that case the test statistic becomes equal to $b$.

Sampling distribution of $F$:
• $F$ distribution with $I - 1$ (df between, numerator) and $N - I$ (df error, denominator) degrees of freedom
Sampling distribution of $t$:
• $t$ distribution with $N - I$ degrees of freedom
Significant?Significant?Significant?Significant?
Two sided:
Right sided:
Left sided:
Two sided:
Right sided:
Left sided:
For test statistic $X^2$:
• Check if $X^2$ observed in sample is equal to or larger than critical value $X^{2*}$ or
• Find $p$ value corresponding to observed $X^2$ and check if it is equal to or smaller than $\alpha$
If $b + c$ is small, the table for the binomial distribution should be used, with as test statistic $b$:
• Check if $b$ observed in sample is in the rejection region or
• Find two sided $p$ value corresponding to observed $b$ and check if it is equal to or smaller than $\alpha$
$F$ test:
• Check if $F$ observed in sample is equal to or larger than critical value $F^*$ or
• Find $p$ value corresponding to observed $F$ and check if it is equal to or smaller than $\alpha$ (e.g. .01 < $p$ < .025 when $F$ = 3.91, df between = 4, and df error = 20)

$t$ Test for contrast two sided:
$t$ Test for contrast right sided:
$t$ Test for contrast left sided:

$t$ Test multiple comparisons two sided:
• Check if $t$ observed in sample is at least as extreme as critical value $t^{**}$. Adapt $t^{**}$ according to a multiple comparison procedure (e.g., Bonferroni) or
• Find two sided $p$ value corresponding to observed $t$ and check if it is equal to or smaller than $\alpha$. Adapt the $p$ value or $\alpha$ according to a multiple comparison procedure
$t$ Test multiple comparisons right sided
• Check if $t$ observed in sample is equal to or larger than critical value $t^{**}$. Adapt $t^{**}$ according to a multiple comparison procedure (e.g., Bonferroni) or
• Find right sided $p$ value corresponding to observed $t$ and check if it is equal to or smaller than $\alpha$. Adapt the $p$ value or $\alpha$ according to a multiple comparison procedure
$t$ Test multiple comparisons left sided
• Check if $t$ observed in sample is equal to or smaller than critical value $t^{**}$. Adapt $t^{**}$ according to a multiple comparison procedure (e.g., Bonferroni) or
• Find left sided $p$ value corresponding to observed $t$ and check if it is equal to or smaller than $\alpha$. Adapt the $p$ value or $\alpha$ according to a multiple comparison procedure
Approximate $C\%$ confidence interval for $\mu_1 - \mu_2$n.a.n.a.$C\%$ confidence interval for $\Psi$, for $\mu_g - \mu_h$, and for $\mu_i$
$(\bar{y}_1 - \bar{y}_2) \pm t^* \times \sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}$
where the critical value $t^*$ is the value under the $t_{k}$ distribution with the area $C / 100$ between $-t^*$ and $t^*$ (e.g. $t^*$ = 2.086 for a 95% confidence interval when df = 20).

The confidence interval for $\mu_1 - \mu_2$ can also be used as significance test.
--Confidence interval for $\Psi$ (contrast):
• $c \pm t^* \times s_p\sqrt{\sum \dfrac{a^2_i}{n_i}}$
where the critical value $t^*$ is the value under the $t_{N - I}$ distribution with the area $C / 100$ between $-t^*$ and $t^*$ (e.g. $t^*$ = 2.086 for a 95% confidence interval when df = 20). Note that $n_i$ is the sample size of group $i$, and $N$ is the total sample size, based on all the $I$ groups.
Confidence interval for $\mu_g - \mu_h$ (multiple comparisons):
• $(\bar{y}_g - \bar{y}_h) \pm t^{**} \times s_p\sqrt{\dfrac{1}{n_g} + \dfrac{1}{n_h}}$
where $t^{**}$ depends upon $C$, degrees of freedom ($N - I$), and the multiple comparison procedure. If you do not want to apply a multiple comparison procedure, $t^{**} = t^* =$ the value under the $t_{N - I}$ distribution with the area $C / 100$ between $-t^*$ and $t^*$. Note that $n_g$ is the sample size of group $g$, $n_h$ is the sample size of group $h$, and $N$ is the total sample size, based on all the $I$ groups.
Confidence interval for single population mean $\mu_i$:
• $\bar{y}_i \pm t^* \times \dfrac{s_p}{\sqrt{n_i}}$
where $\bar{y}_i$ is the sample mean in group $i$, $n_i$ is the sample size of group $i$, and the critical value $t^*$ is the value under the $t_{N - I}$ distribution with the area $C / 100$ between $-t^*$ and $t^*$ (e.g. $t^*$ = 2.086 for a 95% confidence interval when df = 20). Note that $n_i$ is the sample size of group $i$, and $N$ is the total sample size, based on all the $I$ groups.
n.a.n.a.n.a.Effect size
---
• Proportion variance explained $\eta^2$ and $R^2$:
Proportion variance of the dependent variable $y$ explained by the independent variable: \begin{align} \eta^2 = R^2 &= \dfrac{\mbox{sum of squares between}}{\mbox{sum of squares total}} \end{align} Only in one way ANOVA $\eta^2 = R^2.$ $\eta^2$ (and $R^2$) is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population.

• Proportion variance explained $\omega^2$:
Corrects for the positive bias in $\eta^2$ and is equal to: $$\omega^2 = \frac{\mbox{sum of squares between} - \mbox{df between} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}$$ $\omega^2$ is a better estimate of the explained variance in the population than $\eta^2.$

• Cohen's $d$:
Standardized difference between the mean in group $g$ and in group $h$: $$d_{g,h} = \frac{\bar{y}_g - \bar{y}_h}{s_p}$$ Cohen's $d$ indicates how many standard deviations $s_p$ two sample means are removed from each other.
Visual representationn.a.n.a.n.a.
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n.a.n.a.n.a.ANOVA table
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Click the link for a step by step explanation of how to compute the sum of squares.
n.a.n.a.Equivalent toEquivalent to
--
OLS regression with one categorical independent variable transformed into $I - 1$ code variables:
• $F$ test ANOVA is equivalent to $F$ test regression model
• $t$ test for contrast $i$ is equivalent to $t$ test for regression coefficient $\beta_i$ (specific contrast tested depends on how the code variables are defined)
Example contextExample contextExample contextExample context
Is the average mental health score different between men and women?Is there a monotonic relationship between physical health and mental health?Does a tv documentary about spiders change whether people are afraid (yes/no) of spiders?Is the average mental health score different between people from a low, moderate, and high economic class?
SPSSSPSSSPSSSPSS
Analyze > Compare Means > Independent-Samples T Test...
• Put your dependent (quantitative) variable in the box below Test Variable(s) and your independent (grouping) variable in the box below Grouping Variable
• Click on the Define Groups... button. If you can't click on it, first click on the grouping variable so its background turns yellow
• Fill in the value you have used to indicate your first group in the box next to Group 1, and the value you have used to indicate your second group in the box next to Group 2
• Continue and click OK
Analyze > Correlate > Bivariate...
• Put your two variables in the box below Variables
• Under Correlation Coefficients, select Spearman
Analyze > Nonparametric Tests > Legacy Dialogs > 2 Related Samples...
• Put the two paired variables in the boxes below Variable 1 and Variable 2
• Under Test Type, select the McNemar test
Analyze > Compare Means > One-Way ANOVA...
• Put your dependent (quantitative) variable in the box below Dependent List and your independent (grouping) variable in the box below Factor
or
Analyze > General Linear Model > Univariate...
• Put your dependent (quantitative) variable in the box below Dependent Variable and your independent (grouping) variable in the box below Fixed Factor(s)
JamoviJamoviJamoviJamovi
T-Tests > Independent Samples T-Test
• Put your dependent (quantitative) variable in the box below Dependent Variables and your independent (grouping) variable in the box below Grouping Variable
• Under Tests, select Welch's
• Under Hypothesis, select your alternative hypothesis
Regression > Correlation Matrix
• Put your two variables in the white box at the right
• Under Correlation Coefficients, select Spearman
• Under Hypothesis, select your alternative hypothesis
Frequencies > Paired Samples - McNemar test
• Put one of the two paired variables in the box below Rows and the other paired variable in the box below Columns
ANOVA > ANOVA
• Put your dependent (quantitative) variable in the box below Dependent Variable and your independent (grouping) variable in the box below Fixed Factors
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